Linking Premenstrual Syndrome With Salivary  Alpha-Amylase Levels and Stress Levels:  A Cross-Sectional Study

Gabriyah  Hamzah; Aryadi  Arsyad; Elizabet C  Jusuf

doi:10.18502/jfrh.v19i3.20058

Gabriyah Hamzah Hasanuddin University, Makassar, Indonesia
Aryadi Arsyad Department of Physiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Elizabet C Jusuf Department of Obstetrics and Gynecology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

DOI: https://doi.org/10.18502/jfrh.v19i3.20058

Keywords: Salivary Alpha-Amylase; Premenstrual Syndrome; Stress

Abstract

Objective: This study aims to analyze the correlation between salivary alpha-amylase (sAA) and stress in adolescents with premenstrual syndrome (PMS), and to assess the predictive ability of sAA levels and stress for PMS.

Materials and methods: Sixty-two adolescents with and without PMS (31 each) were grouped based on their PMS status, measured using the Shortened Premenstrual Assessment Form (SPAF). Stress was measured using the Kessler Psychological Distress Scale (K10). The levels of sAA were measured using Enzyme-Linked Immunosorbent Assay (ELISA). Data were analyzed using the Pearson correlation test, Receiver Operating Characteristic (ROC) curve analysis, and multiple linear regression analysis.

Results: Stress and PMS expressed a significant positive correlation (p=0.001; r=0.66). sAA levels and PMS showed a significant positive correlation (p<0.05; r=0.42). The level of sAA in adolescents with PMS was 23.28±12.02 ng/mL, almost twice higher than in adolescents without PMS (12.10±7.5 ng/mL). The cut-off value of sAA level on PMS was ≥15.02 ng/mL with a sensitivity and specificity of 67.7%. The level of sAA and stress were significantly able to predict PMS (p<0.001). The correlation value of SAA levels and stress with PMS is positive (r=0.705), suggesting they can predict PMS by
49.7% (R²=0.497).

Conclusion: There is a positive relationship between sAA levels and stress in adolescents with PMS. SAA levels and stress were able to predict PMS in adolescents with an accuracy of 49.7%.