How Does Platelet-rich Plasma Injection in Ovaries  of Poor Responders Affect the Retrieved Oocytes,  and Anti Mullerian Hormone: A Clinical Trial

Ensieh Shahrokh Tehraninejad; Maryam Olsadat Razavi; Azadeh Tarafdari Menshadi; Mamak Shariat; Saiedeh Shahsavari; Fedyeh Haghollahi; Elham Azimi Nekoo

doi:10.18502/jfrh.v17i3.13539

Ensieh Shahrokh Tehraninejad Vali-E-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Maryam Olsadat Razavi Department of Obstetrics & Gynecology, Ardebil University of Medical Sciences, Ardebil, Iran
Azadeh Tarafdari Menshadi Vali-E-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Mamak Shariat Maternal, Fetal & Neonatal Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Saiedeh Shahsavari Department of Obstetrics and Gynecology, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Fedyeh Haghollahi Vali-E-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Elham Azimi Nekoo Jacobi Medical Center / Albert Einstein University, New York, United States

DOI: https://doi.org/10.18502/jfrh.v17i3.13539

Keywords: Platelet-Rich Plasma; Anti-Mullerian Hormone; Ovarian Reserve; In Vitro Fertilization

Abstract

Objective: Platelet Rich Plasma (PRP) is proposed to have important role in cell division and proliferation, angiogenesis and health. This study evaluates the effect of a single injection of autologous PRP on ovarian response markers in women with poor ovarian response (POR).

Materials and methods: This non-randomized clinical trial was conducted between August 2020 and September 2021. Fifty six women with Bologna criteria for POR willingly chose to participate in one of the following groups: PRP for one cycle in the time of oocyte pickup (OPU) (intervention group, n= 34) or control group (n=22).The primary outcomes were: number and quality of oocytes in coming 2 cycles of ICSI, and Anti Mullerian Hormone (AMH) level two months after PRP injection. The secondary outcomes were the number and quality of embryos and chemical pregnancy rate after embryo transfer.

Results: A total of 45 participants continued the study, of which 23 were in the intervention group and 22 in control group. There were no demographic differences between two groups. At a two cycle follow up, PRP group experienced a significant improvement in AMH level and there was no respective change in control group. In one year follow up the overall pregnancy rates were same in both groups (3% Vs. 0, p=.60), while there was no difference in cumulative number and quality of embryos.

Conclusion: PRP injection can improve ovarian reserve marker without adverse effects. Further evidence is required to evaluate the impact of PRP on assisted reproduction outcomes.