Prevalence, Molecular Identification, Antimicrobial Resistance, and Disinfectant Susceptibility of Listeria innocua Isolated from  Ready-to-Eat Foods Sold in Johannesburg, South Africa

H.H.   Makumbe; F.T.  Tabit; B.C.  Dlamini

doi:10.18502/jfqhc.8.3.7200

H.H. Makumbe Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, 55 Beit St, Doornfontein, Johannesburg, 2028, Johannesburg, South Africa
F.T. Tabit Department of Life and Consumer Sciences, University of South Africa, Cnr Christiaan de Wet Road and Pioneer Avenue, Florida, Roodepoort 1710, Johannesburg, South Africa
B.C. Dlamini Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, 55 Beit St, Doornfontein, Johannesburg, 2028, Johannesburg, South Africa

DOI: https://doi.org/10.18502/jfqhc.8.3.7200

Keywords: Listeria innocua Drug Resistance, Microbial Disinfectants Polymerase Chain Reaction Fast Foods South Africa

Abstract

Background: Food contamination with Listeria spp. can occur at all stages of the food chain. The aim of this research was to investigate the prevalence, molecular identification, antimicrobial resistance, and disinfectant susceptibility of Listeria innocua isolated from Ready-To-Eat (RTE) foods sold in Johannesburg, South Africa.

Methods: Eighty RTE foods were collected from Johannesburg, South Africa. The 16S rRNA region of L. innocua isolates was amplified, sequenced, and identified using Basic Alignment Search Tool (BLAST). The antimicrobial resistance and disinfectant susceptibility (against four commercial disinfectants) of the isolates were evaluated using disk diffusion and microdilution assays. Data were statistically analyzed using SPSS v. 23.0.

Results: Listeria strains revealed a high 16S rRNA gene sequence analogy to L. innocua of between 98-99%. The overall prevalence of L. innocua was 21.3% (17 out of 80) in the RTE food samples. Most isolates were susceptible to the studied commercial disinfectants. All the L. innocua isolates from food sources were found to be resistant to ampicillin and cephalothin, while 83 and 74% of isolates were resistant to colistin
sulphate and sulphatriad.

Conclusion: Prevalence of L. innocua was considerable in the RTE food samples sold in Johannesburg, South Africa. The L. innocua isolates showed high antibiotic resistance against ampicillin, cephalothin, colistin sulphate, and sulphatriad.