Investigation of TEM and SHV Beta-Lactamase Genes in  Escherichia coli Isolated from Strawberry Samples in Sanandaj, Iran

M.M.  Soltan Dallal; H.  Abdolmaleki; R.  Ramazanzadeh; R.   Mazaheri Nezhad Fard

doi:10.18502/jfqhc.8.3.7199

M.M. Soltan Dallal Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
H. Abdolmaleki Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
R. Ramazanzadeh Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
R. Mazaheri Nezhad Fard Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/jfqhc.8.3.7199

Keywords: Escherichia coli beta-Lactamases Drug Resistance, Microbial Polymerase Chain Reaction Fragaria Iran

Abstract

Background: When animal manures are used, food products may include pathogenic bacteria, especially Escherichia coli. The major aim of the current study was to investigate TEM (bla_TEM) and SHV beta-lactamase (bla_SHV) genes in E. coli isolated from strawberry samples in Sanandaj, Iran.

Methods: In this study, 150 strawberry samples were collected from farms (traditional), greenhouses, and packages in Sanandaj, Iran. E. coli contamination was done using routine culture methods. Then, isolates were investigated for Extended-Spectrum Beta-Lactamase (ESBL) production and bla_TEM or bla_SHV genes using phenotypic and genotypic methods, respectively.

Results: The most susceptibility and resistance of E. coli to antibiotics were related to chloramphenicol and trimethoprim/sulfamethoxazole, respectively. Out of 21 isolates of E. coli, eight were resistant to ceftazidime and cefotaxime; from which, six isolates were ESBL-producer. Furthermore, Polymerase Chain Reaction (PCR) analysis of six ESBL- producing E. coli isolates showed that four isolates included bla_TEM gene, while no
isolates included bla_SH_V gene.

Conclusion: In this study, multiple antibiotic resistance patterns were seen in E. coli isolates, especially ESBL patterns in E. coli isolated from strawberries produced in Iran.