In Vitro Comparative Analysis of Antioxidant Activities in Ethanolic  Extracts of Centella Asiatica Linn., Ocimum Basilicum, Ocimum  Sanctum, and Camellia Sinensis

Nazifa  Javaid; Meghanand T  Nayak; Anjali   Nayak; Rezhat  Abass; Mohd. Zanul  Abedeen; Milind Sharad  Pande

doi:10.18502/jcr.v13i1.21477

Nazifa Javaid Department of Oral Pathology and Microbiology, Institute of Dental Studies and Technologies, Modinagar, India.
Meghanand T Nayak Department of Oral Pathology and Microbiology, Surendera Dental College and Research Institute, Sriganganagar, Rajasthan, India.
Anjali Nayak Department of Oral Medicine and Radiology, Surendera Dental College and Research Institute, Sriganganagar, Rajasthan, India.
Rezhat Abass Department Oral Pathology and Microbiology, Maulana Azad Institute of Dental Sciences, New Delhi, India.
Mohd. Zanul Abedeen Department of Oral Pathology and Microbiology, Teerthanker Mahaveer Dental College and Research Centre, Moradabad, India.
Milind Sharad Pande IIMT College of Medical College, Department of Pharmacy, Meerut, India.

DOI: https://doi.org/10.18502/jcr.v13i1.21477

Keywords: Antioxidants; Centella asiatica; Camellia sinensis; Ocimum basilicum; Ocimum sanctum; Oxidative stress.

Abstract

Introduction: To comparatively evaluate the antioxidant potential of ethanolic extracts of Centella asiatica Linn. (CA), Ocimum basilicum Linn. (OB), Ocimum sanctum (OS), and Camellia sinensis (CS) using multiple in vitro assays.

Materials and Methods: Ethanolic extracts of CA, OB, OS, and CS were prepared and subjected to in vitro antioxidant analysis, including total antioxidant capacity, DPPH radical scavenging, nitric oxide radical scavenging, reducing power, and superoxide anion radical scavenging assays using UV spectrophotometry. Antioxidant activities were recorded at concentrations up to 1000 µg/ml. All assays were performed across a concentration range of 10–1000 µg/ml using serial dilutions.

Results: Total antioxidant activity (109.40 ± 0.15 % inhibition), DPPH scavenging (107.88 ± 9.32 % inhibition), nitric oxide scavenging (98.27 ± 9.48 % inhibition), reducing power (absorbance: 93.12 ± 9.12 at 700 nm), and superoxide scavenging (88.93 ± 8.14 % inhibition). The differences between groups were statistically significant (p < 0.001).

Conclusion: CA demonstrated superior antioxidant activity compared to OB, OS, and CS. Its high antioxidant potential suggests its promise as a natural therapeutic agent for preventing oxidative stress–related diseases.