Invasive Mucormycosis and the Importance of Molecular Diagnostics

Abdolreza  Mohamadnia; Amir Jalal  Abbasi; Farnoush  Mohammadi; Seyedeh Zahra  Fotook Kiae; Mona  Mohajeri Tehrani; Somaieh  lookzadeh; Fatemeh  Yassari; Shadi  Shafaghi; Farnaz  Ahmadi; Naghmeh  Bahrami

doi:10.18502/jcr.v11i2.17026

Abdolreza Mohamadnia Chronic Respiratory Diseases Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Amir Jalal Abbasi Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
Farnoush Mohammadi Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
Seyedeh Zahra Fotook Kiae Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
Mona Mohajeri Tehrani Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
Somaieh lookzadeh Chronic Respiratory Diseases Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Fatemeh Yassari Chronic Respiratory Diseases Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Shadi Shafaghi Lung Transplantation Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Farnaz Ahmadi Lung Transplantation Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Naghmeh Bahrami Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/jcr.v11i2.17026

Keywords: Mucormycosis, diagnosis, Real-time PCR

Abstract

introduction: Mucormycosis, an infection, with a high death rate, requires understanding its symptoms, diagnosis methods and treatment options due, to its growing occurrence. The research employs a molecular method to evaluate the diagnosis of the mucormycosis.

Methods and material: In this study, we obtained 30 samples from patients undergoing diagnosis and conducted DNA extraction. Additionally, DNA extraction was carried out on 30 tissue samples suspected of infection in paraffin blocks. Subsequently, PCR and Real-time PCR were performed using targeted primers, for mucormycosis followed by analysis of the results.

Results: In the research findings among 30 liquid samples 8 tested positive, for mucor using the PCR method. 10 using the Real-time PCR method. Similarly, out of 30 tissue samples, 9 cases showed mucor presence with the PCR method and 11 cases, with the Realtime PCR method.

Conclusion: In this study, real-time PCR and PCR techniques showed promising and faster results in detecting Mucor than the culture approach. The molecular methods provided results that could be of great use for scenarios.