The cytotoxic effect of 4 different wavelengths of Low-level laser therapy on squamous cell carcinoma – An invitro study

Arghavan Tonkaboni; Alten Tatar; Kimia Hafezi  Motlagh; Reza Fekrazad

doi:10.18502/jcr.v11i1.16478

Arghavan Tonkaboni Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Alten Tatar International Campus, School of Dentistry, Tehran University of Medical Science, Tehran, Iran.
Kimia Hafezi Motlagh Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Reza Fekrazad Radiation Sciences Research Center, Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran.

DOI: https://doi.org/10.18502/jcr.v11i1.16478

Keywords: Low-Level Laser Therapy, LLLT, Head and Neck Squamous Cell Carcinomas, HNSCC

Abstract

Background: Low-level laser therapy is a noninvasive method with the potential ability to change the balance of cell mediators and gene expressions. It affects cellular function resulting in beneficial clinical effects. This study aims to assess the effect of low-level light therapy (LLLT) using four different laser wavelengths on oral carcinoma cell viability in vitro.

Methods and Material: HN5 human head and neck squamous cell carcinoma cell lines (HNSCC) were cultured and irradiated using four wavelengths of blue (485 nm), green (532nm), red (660 nm), and near Infra-red (810 nm) in a continuous mode with a dose of 1 J/cm² (0.1W, 10sec) every 24hours for five consecutive days. Cell viability was assessed by evaluating mitochondrial activity by MTT assay.

Results: All the wavelengths resulted in reduced viability of these cells compared to the controls. (P<0.05) There were statistically significant differences in cell viability between different wavelengths (P<0.001). The 810 nm laser irradiation showed the highest percentage of cell survival (55.92%) while 660 nm induced the lowest (36.02%).

Conclusion: Different laser wavelengths may result in different effects on irradiated cells and red irradiation showed the lowest cell viability and the infrared laser had the highest cell viability results.