Comparing Salivary miR-125 and miR-30  Expression in Oral Squamous Cell Carcinoma to Healthy Individuals

Naghmeh bahrami; Marziyeh   Poorahmad; Farzaneh Hosseini; Farnoush  Mohammadi; Amir  Rasouli; Mona  Mohajeri Tehrani; Masoume  Farhangiyan; Abdolreza  Mohamadnia

doi:10.18502/jcr.v10i4.15307

Naghmeh bahrami Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Marziyeh Poorahmad Department of Genetic, School of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Farzaneh Hosseini Department of Microbiology, School of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Farnoush Mohammadi Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Amir Rasouli Department of Biology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.
Mona Mohajeri Tehrani Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Masoume Farhangiyan Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Abdolreza Mohamadnia Chronic Respiratory Diseases Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/jcr.v10i4.15307

Keywords: oral squamous cell carcinoma, miR-125, miR-30, biomarker

Abstract

Introduction: OSCC (Oral squamous cell carcinoma) accounts for approximately 90% of all oral malignancies and is usually diagnosed at advanced stages. This study investigates changes in miR-125 and miR-30 expression in relation to the clinical findings of oral cavity cancer and their possible use as an early diagnostic tool.

Method and Material: A population of 30 individuals with oral squamous cell carcinoma and 30 healthy individuals was studied, and the mean age of the two groups was compared using a t-test, with no significant difference found in terms of age so age will not be an interfering factor in this study. The levels of these two biomarkers (miR-125 and miR-30) were measured and evaluated using real-time PCR technique.

Results: After evaluating the results of the real-time PCR technique, it was found that miR-125 was positive in 25 out of 30 patients, while it was positive in 5 out of 30 healthy individuals (p-value ≤0.001). miR-30 was a positive biomarker in 10 out of 30 patients. The amount of this biomarker in the group of healthy individuals was 26 out of 30 (p-value < 0.001).

CONCLUSION: The miR-125 profile is upregulated in the saliva of OSCC cases, whereas the miR-30 profile is downregulated in the aforementioned patients compared with the healthy group. Therefore, the measurement of miR-125 and miR-30 may be a protentional diagnostic test to identify OSCC. We suggest more extensive studies with a larger sample size to support this claim.