The Effects of Three ABCG2 Polymorphisms on Outcome of Central Nervous System Relapses in Iranian Children With Acute Lymphoblastic Leukemia Receiving High Dose Methotrexate

Raziye Karamikhah; Negar Azarpira; Soheila Zareifar; Ali Dehshahri; Soha Namazi; Mohammad Hossein Anbardar; Iman Karimzadeh

doi:10.18502/acta.v59i3.5783

Raziye Karamikhah
Negar Azarpira
Soheila Zareifar
Ali Dehshahri
Soha Namazi
Mohammad Hossein Anbardar
Iman Karimzadeh

DOI: https://doi.org/10.18502/acta.v59i3.5783

Keywords: : Methotrexate; ATP-binding cassette transporter G2 (ABCG2); Central nervous system (CNS) relapse; Childhood acute lymphoblastic leukemia

Abstract

Methotrexate (MTX) is the main drug for the treatment of childhood acute lymphoblastic leukemia (ALL). ABCG2 pump is the main transporter of MTX on BBB. Our aim was to investigate the possible relationship between three polymorphisms of the ABCG2 gene, and isolated CNS relapses in Iranian children with ALL receiving high dose MTX. Genotyping of three polymorphisms of the ABCG2 gene, including G34A, C376T, and C421A, was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for 56 patients. A high frequency of C376T CT genotype was observed among the patients. There was no significant association between C376T and C421 and isolated CNS relapse (P>0.05). C376T and C421A polymorphisms are not associated with isolated CNS relapse in childhood ALL.