Effect of Green Peas Protein Hydrolysate on Antihyperlipidemia and Antinephrotoxicity of Gentamicin-Induced Wistar Rats

Abstract

Green peas protein hydrolysate is used in the treatment of kidney disease. Nephrotoxicity of gentamicin (GM) is known to be involved in kidney changes, dyslipidemia, and hematology. The aim of the present study is to evaluate the effects of three doses of green peas protein hydrolysate bromelain (GPPHB) as antinephrotoxicity and antihyperlipidemia on gentamicin (GM)-induced Wistar rats as an effort to find an effective dose for kidney diseases therapy. Nephrotoxicity was induced in female Wistar rats through intraperitoneal administration of GM 80 mg/kg/day for seven days. Nine treatment groups (n=6) were monitored to determine the effects of the concurrent administration of green peas protein hydrolysate at a dose of 50, 100, and 200 mg/kg/day given with GM toward kidney damage using serum creatinine and blood urea nitrogen as indicators. The parameters for nephrotoxicity of GM were kidney organ index (OI), histopathological: cloudy swelling tubular degeneration, nucleus necrosis, and hyaline cast; hematology profiles; and for hyperlipidemia were total cholesterol, low-density lipoprotein (LDL), and triglyceride. After 28 days of treatments, GPPHB at the dose of 50 mg/kgBW has a good effect on kidney OI, whereas, at a dose of 200 mg/kgBW, GPPHB significantly protects rat kidneys from GM-induced histopathological changes. The GPPHB dose of 100 and 200 mg/kgBW significantly lowered the total cholesterol, LDL, and triglyceride levels but had no significant effect on hematology profiles. Three doses of GPPHB had antihyperlipidemia and antinephrotoxicity effects in GM-induced nephrotoxicity Wistar rats.