Correlation Between ncRNA Gene Profiles and NEIL1 Expression in Breast Cancer

Samaa M.  Sayel; Owayes M.  Hamed

doi:10.18502/acta.v64i4.21989

Samaa M. Sayel Department of Biology, Collage of Education for Pure Sciences, University of Al-Hamdaniya, Ninevah, Iraq
Owayes M. Hamed Department of Biology, College of Sciences, University of Mosul, Mosul, Iraq

DOI: https://doi.org/10.18502/acta.v64i4.21989

Keywords: Breast cancer; Base excision repair; miRNA3912-3p; snoRNA SNHG7; lncRNA BC069792; Neil1 gene

Abstract

Endonuclease VIII-like 1, a crucial DNA repair enzyme encoded by the Neil1 gene, is essential for shielding the genome from oxidative damage. It is an essential part of the Base Excision Repair (BER) pathway, which fixes minor but dangerous DNA defects. We describe how the Neil family contributes to cancer’s harmful biological characteristics. The aim is to detect some types of ncRNA genes that can be used as molecular markers for breast cancer. The methodology involves detecting the gene expression levels of the Neil1 gene and several ncRNA genes, including miRNA‑3912‑3p, snoRNA SNHG7, and lncRNA BC069792, using the qPCR technique. The results showed increased expression levels of the Neil1 gene (2.435), miRNA‑3912‑3p (12.34), lncRNA BC069792 (6.11), and snoRNA SNHG7 (5.08) in patients with biopsy samples, while decreased expression levels were observed for all genes in blood samples compared with healthy cases. The conclusion from this study suggests that miRNA‑3912‑3p may be considered a regulatory factor for the Neil1 gene and a potential biomarker for breast cancer.