Seminal Histone Deacetylase, Fructose and Serum Reproductive Hormones as Diagnostic Marker in Sub-Groups of Infertile Males

Wasiu E Olooto; Hammed A Adenusi; Joseph O Soola; Monisola A Ayodele; Frank E Agidigbi

doi:10.18502/acta.v61i4.13170

Wasiu E Olooto Department of Chemical Pathology and Immunology, Olabisi Onabanjo University, Ago-Iwoye, Ogun, Nigeria
Hammed A Adenusi Department of Chemical Pathology and Immunology, Olabisi Onabanjo University, Ago-Iwoye, Ogun, Nigeria
Joseph O Soola Department of Physiology, Olabisi Onabanjo University, Ago-Iwoye, Ogun, Nigeria
Monisola A Ayodele Department of Chemical Pathology and Immunology, Olabisi Onabanjo University, Ago-Iwoye, Ogun, Nigeria
Frank E Agidigbi School of Medical Laboratory Technology, Baptist Medical Center, Saki, Nigeria

DOI: https://doi.org/10.18502/acta.v61i4.13170

Keywords: Seminal fructose; Histone deacetylases; Infertile men; Adiposity; Gonadotropins

Abstract

Accurate diagnosis of the cause of infertility assists in the choice of treatment modalities and amelioration of the associated psychosocial problems. The research was carried out using 75 infertile males and 75 males with proven fertility as controls. The anthropometrics (weight, height) were measured and body mass index (BMI) computed. Venous blood was collected from each participant, allowed to clot, and centrifuged to obtain the serum which was analysed for testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH) concentrations. Semen was collected by masturbation and analysed for sperm quality, seminal fructose concentration, and histone deacetylase (HDAC) activities. A non-significant difference (P>0.05) in weight, height, and BMI; a significant increase (P<0.05) in ejaculated volume, serum LH, FSH, seminal fructose concentrations and HDAC activities; and a decrease in sperm count, sperm motility, serum testosterone concentration was observed among subgroups of infertile men. A direct and significant correlation exists between seminal HDAC activities and fructose concentration. Also, an inverse non-significant correlation exists between HDAC activities and spermatozoa motility. Base on the result obtained from this study, it can be concluded that measuring seminal fructose and HDAC activities in addition to routine biochemical and biophysical parameters will assist in diagnostic work up in subgroups of male infertility.