Therapeutic Effects of Aloe vera (L.) Burm.f. and Vitis vinifera L. Combination Cream on Wound Healing in Second-Degree Burn Model in Rats: Quantiﬁcation of Compounds and VEGF & TGFβ Gene Expression

Ardeshir Moayeri; Kholoud Ramz; Elahe Karimi; Monireh Azizi; Naser Abbasi; Ali Aidy; Mahmoud Bahmani

doi:10.18502/tim.v7i1.9063

Ardeshir Moayeri Department of Anatomy, Medical School, Ilam University of Medical Sciences, Ilam, Iran
Kholoud Ramz Department of Anatomy, Medical School, Ilam University of Medical Sciences, Ilam, Iran
Elahe Karimi Biotechnology and Medicinal Plants Research Center, Medical School, Ilam University of Medical Sciences, Ilam, Iran
Monireh Azizi Department of Anatomy, Medical School, Ilam University of Medical Sciences, Ilam, Iran
Naser Abbasi Biotechnology and Medicinal Plants Research Center, Medical School, Ilam University of Medical Sciences, Ilam, Iran
Ali Aidy Biotechnology and Medicinal Plants Research Center, Medical School, Ilam University of Medical Sciences, Ilam, Iran
Mahmoud Bahmani Biotechnology and Medicinal Plants Research Center, Medical School, Ilam University of Medical Sciences, Ilam, Iran

DOI: https://doi.org/10.18502/tim.v7i1.9063

Keywords: Aloe vera; Vitis vinifera; Second-degree burns; HPLC; VEGF

Abstract

Finding more efficient agents with fewer side effects for the treatment of burns has been a concern for researchers. The current study aims to assess Aloe vera (L.) Burm.f. and Vitis vinifera L. extract combination (AVEC) effects on wound healing in rats compared with silver sulfadiazine (SSD). Identification of individual polyphenols of the plant extracts was performed by HPLC. The animals were randomly divided into twelve groups. Standard second-degree burn wounds were induced on the back. Groups were treated with Aloe vera (A.V) and Vitis vinifera (V.V) creams at concentrations of 0.5, 1, 1.5, and 2% with ED₅₀ values of 1.5 ± 0.02 and 1.4 ± 0.08 %, respectively. The other group was treated with an AVEC cream (1.5%). The samples of burned skin tissue were collected from the rats for histopathological examination. To evaluate the expression of VEGF and TGFβ1, the real time-PCR method was used. Kaempferol was only detected in Aloe. This study revealed that AVEC cream exhibits significant wound healing activity. VEGF and TGFβ1 had a significant increase in the AVEC. Based on our findings, AVEC cream can be a therapy of choice for burn injuries.