Effect of Biochanin A on Serum Nesfatin-1 Level in STZ Induced Type 1 Diabetic Rat

Maryam Eskandari Mehrabadi; Zahra Salemi; Farshad  Eskandari Mehrabadi

doi:10.18502/tim.v6i2.6777

Maryam Eskandari Mehrabadi Students Research Committee, Arak University of Medical Sciences, Arak, Iran
Zahra Salemi Department of Biochemistry, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
Farshad Eskandari Mehrabadi Islamic Azad University, Ayatollah Amoli Branch, Amol, Mazandaran, Iran

DOI: https://doi.org/10.18502/tim.v6i2.6777

Keywords: Nesfatin-1; Biochanin A; Diabetes; Rat

Abstract

recognized as a regulatory peptide, which can increase insulin sensitivity and affect glucose metabolism. In this study, effects of BCA, on serum nesfatin-1 level were examined in rats with streptozotocin (STZ)-induced diabetes. We randomly divided 30 male Wistar rats into 2 control (6 rats per group) and 3 diabetic groups. Type I diabetes was induced using STZ (55 mg/kg bw) injection. Group 1 received 0.5% DMSO; while group 2 received 10 mg/kg body weight of BCA; group 3 (diabetic controls) received 0.5% DMSO; group 4, 10 mg/kg of BCA; and group 5, 15 mg/kg of BCA. The levels of serum insulin, nesfatin-1, and fasting blood glucose (FBG) were determined after 42 days. The serum insulin level increased; while FBG level significantly decreased in the BCA treatment groups. Both treatment groups had increased nesfatin-1 levels in comparison with the control groups; however, the difference was only significant in group 5 (p value < 0.05). Considering insulin and nesfatin-1 induction, BCA has potential hypoglycemic effects. Oral administration of 15 mg/kg BW of BCA showed greater efficacy than 10 mg/kg bw of BCA.