In-vitro Antibacterial Effects of Aqueous, Ethanolic, Methanolic, Acetone and Hydro Ethanolic Extracts of Achillea Millefolium on Standard Klebsiella, S. pyogenes, and Oral Bacterias Strains

  • Negar Fallah
  • Sanaz Namazi
  • Negar Balmeh
  • Samira Mahmoudi
  • Fereshteh Mirzaei Poor
Keywords: Achillea millefolium, In-vitro Antibacterial Effects, Klebsiella


Introduction: So far, a lot of attempts have been carried out to find antimicrobial compounds. In this study, it was also tried to investigate the antibacterial effects of Achillea millefolium on standard KlebsiellaSpyogenes, and oral bacterias strain.

Material and Methods: The aerial parts of Achillea millefolium were used and the aqueous, ethanolic, methanolic, acetone and hydroethanolic extracts were prepared. After the preparation of standard strains of KlebsiellaS. pyogenes, and oral bacterias and sterilization of extracts by the Millipore filter, the antibacterial effects of these extracts on the mentioned microorganisms were assessed by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and well diffusion at the concentration 50 mg/ml. The test was repeated three times for each bacterium.

Results: It was exposed that aqueous extract of Achillea millefolium had the most distinguished antimicrobial effects against all studied strains and methanolic extract had antimicrobial effects only on S. pyogenes. MIC and MBC of effective extracts were the basic concentration (50mg/ml), and non-growth zone was not observed in other serial dilution in case of all bacteria.

Conclusion: The Achillea millefolium can be admitted as an antibacterial medicinal herb. Thus, it can be concluded that after evaluating their effects in vitro, Achillea millefolium can be utilized as an alternative to the routine chemical drugs.