Effect of Salicylic acid on Phenols and flavonoids content and DPPH scavenging activity in cell suspension culture of Iranian sodab (Ruta graveolens)

Yaser  Sharifi; Valiollah   Ghasemi Omran; Toktam Sadat    Tavabe Ghavami; Ali  Nematzadeh Gharakhili; Mohammad Ali Ebrahimzadeh

doi:10.18502/tbsrj.v1i4.2250

Yaser Sharifi
Valiollah Ghasemi Omran
Toktam Sadat Tavabe Ghavami
Ali Nematzadeh Gharakhili
Mohammad Ali Ebrahimzadeh

DOI: https://doi.org/10.18502/tbsrj.v1i4.2250

Keywords: Salicylic acid, Phenolic content, flavonoids, DPPH, Cell suspension culture, Ruta graveolens, Sodab

Abstract

Ruta graveolens, locally called sodab or sadab, is a known medicinal plant in north of Iran. The plant is a source for the production of secondary metabolites such as Glycosides, lignins, alkaloids, Coumarins, flavonoids and phenolic acids. In this work, the effect of salicylic acid on phenols and flavonoids contents of R. graveolens were investigated in the cell suspension culture. A significant enhancement in total phenolic and flavonoids contents were observed in salicylic acid treated samples. The results revealed that salicylic acid (10 mg/ml) showed as maximum as 3.14 fold enhancement in total flavonoid content (40.35 mg/g) and salicylic acid (20 mg quercetin equivalent/g of extract powder) showed as maximum as 18.33 fold improvement in total phenolic content (438.75 mg gallic acid equivalent/g of extract powder). Also in this study, salicylic acid (10 mg/ml) showed as maximum as 3.55 fold increase in DPPH radical-scavenging activity compared to control. These results suggest that the positive effect of salicylic acid on phenols and flavonoids content and DPPH scavenging activity in cell suspension culture. This effect was dose dependent.