Phyto-Fabrication of Silver Nanoparticles Using  Typha azerbaijanensis Aerial Part and Root Extracts

Amir  Mirzaie; Farzad  Badmasti; Hedieh  Dibah; Shadi  Hajrasouliha; Fatemeh  Yousefi; Romina  Andalibi; Aliasghar  Bagheri Kashtali; Amir Hossein  Rezaei; Ronak  Bakhtiatri

doi:10.18502/ijph.v51i5.9425

Amir Mirzaie Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran
Farzad Badmasti Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
Hedieh Dibah Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
Shadi Hajrasouliha Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
Fatemeh Yousefi Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
Romina Andalibi Department of Biology, Medical Science Branch, Islamic Azad University, Tehran, Iran
Aliasghar Bagheri Kashtali Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
Amir Hossein Rezaei Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran
Ronak Bakhtiatri Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/ijph.v51i5.9425

Keywords: Typha azerbaijanensis; Silver nanoparticle; Antibacterial; Anticancer; Antioxidant; Apoptosis

Abstract

Background: Silver nanoparticles (AgNPs) were phyto-synthesized using Typha azerbaijanensis aerial part and root extracts, and their biological activities were investigated.

Methods: This study was conducted in the Science and Research Branch, Islamic Azad University, Tehran, Iran in 2019. In this experimental study, silver nanoparticles (AgNPs) were phyto-synthesized and the physicochemical properties of AgNPs were determined using UV-Vis (UV-Vis) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy. Antibacterial and anticancer activity of synthesized AgNPs was determined using microdilution assay, and MTT 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) methods, respectively. The apoptotic effects of AgNPs were investigated using Real-Time PCR and flow cytometry techniques.

Results: Morphological analysis of the synthesized AgNPs confirmed the spherical shape of AgNPs with an average size of 10.67 to 16.69 nm. The FTIR spectrum confirmed the presence of phytochemicals from T. azerbayenensis extract at the AgNP surface. Antibacterial experiments showed that phyto-fabricated AgNPs had significant antibacterial activity against Gram-negative bacteria. The AgNPs were significantly cytotoxic against breast cancer cell line (MCF-7) through induction of apoptosis.

Conclusion: The phyto-synthesized AgNPs had biological activities could be useful in pharmaceutical applications.