The Effect of SLC2A3 Expression on Cisplatin Resistance of Colorectal Cancer Cells

Yan  Li; Hailong  Lei; Ming  Zhang; Guangming Wu; Caiyun  Guo; Zijing  Yang; Jingting  Zhang; Yuan  Wang; Jianbin  Zhu; Yongzhe Du

doi:10.18502/ijph.v50i12.7941

Yan Li Digestive Internal, The People’s Hospital of Wuhai, Wuhai, P.R. China
Hailong Lei Internal Medicine-Oncology, The People’s Hospital of Wuhai, Wuhai, P.R. China
Ming Zhang General Surgery, The People’s Hospital of Wuhai, Wuhai, P.R. China
Guangming Wu General Surgery, The People’s Hospital of Wuhai, Wuhai, P.R. China
Caiyun Guo Digestive Internal, The People’s Hospital of Wuhai, Wuhai, P.R. China
Zijing Yang Digestive Internal, The People’s Hospital of Wuhai, Wuhai, P.R. China
Jingting Zhang Clinical Laboratory, The People's Hospital of Wuhai, Wuhai, P.R. China
Yuan Wang Clinical Laboratory, The People's Hospital of Wuhai, Wuhai, P.R. China
Jianbin Zhu Digestive Internal, The People’s Hospital of Wuhai, Wuhai, P.R. China
Yongzhe Du Digestive Internal, The People’s Hospital of Wuhai, Wuhai, P.R. China

DOI: https://doi.org/10.18502/ijph.v50i12.7941

Keywords: Colon cancer; MiRNA; Cisplatin resistance

Abstract

Background: To study the molecular mechanism of cisplatin chemotherapy resistance in colorectal cancer cells and to explore the effect of miRNA in regulating the expression of glucose transporter 3 (SLC2A3) and the proliferation and migration of colon cancer cells.

Methods: All samples were obtained from the People’s Hospital of Wuhai, Wuhai, China between June 2019 and June 2020. Real-time quantitative PCR (qRT-PCR) was carried out to check the expression of miR-103a in these cell lines. Western blotting and Luciferase reporter gene detection confirmed the regulation of the miR-103a/SLC2A3 axis. Western blotting detected the activation of SLC2A3, caspased-9 and -3.

Results: The expression of SLC2A3 protein in colon cancer cell lines was significantly higher than that of normal colon cancer cells, while the expression of SLC2A3 miRNA showed no significant difference (P<0.05). Then, through clone formation analysis, SLC2A3 was closely related to the proliferation of human colon cancer cells. Functional recovery experiments showed that increasing the expression of miR-103a could reverse the abnormal proliferation caused by overexpression of SLC2A3.

Conclusion: Overall, miR-103a can inhibit the proliferation of human colon cancer cells by targeting SLC2A3, and this result will provide a potential target for the treatment of colon cancer.