Upregulation of miRNA-155 in Nasopharyngeal Carcinoma Pa-tients

Thuan Duc  Lao; Minh Trong  Nguyen; Dung Huu  Nguyen; Thuy Ai  Huyen Le

doi:10.18502/ijph.v50i8.6810

Thuan Duc Lao Department of Pharmaceutical and Medical Biotechnology, Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam
Minh Trong Nguyen Cho Ray Hospital, Ho Chi Minh City, Vietnam
Dung Huu Nguyen University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam
Thuy Ai Huyen Le Department of Pharmaceutical and Medical Biotechnology, Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam

DOI: https://doi.org/10.18502/ijph.v50i8.6810

Keywords: Mirn155 microRNA; Nasopharyngeal carcinoma; Vietnamese

Abstract

Background: The upregulation of miRNA-155 (miR-155) has been associated with oncogenesis of many human tumors, including nasopharyngeal carcinoma (NPC). However, the profile of miR-155 in Vietnamese NPC patients has not been investigated. The current study aimed to evaluate the miR-155 expression and assess whether miR-155 is a potential biomarker for diagnosis of NPC in Vietnamese patients.

Methods: In current case-control study, total of RNA was isolated from 60 biopsy NPC samples and 60 non-cancerous swab samples were analyzed by Reverse-transcription PCR, qualitative Real-time PCR.

Results: The frequency of miR-155 detection were 78.33%, 15.0% in NPC and non-cancerous samples (P<0.05), respectively. The miR-155 expression level was 4.92 times higher in tumor samples than non-cancerous sample.

Conclusion: Taken together, miR-155 in NPC was upregulated. It may serve as a potential biomarker for NPC in the Vietnamese population.