Phylogeography and Genetic Diversity of Human Hydatidosis in Bordering the Caspian Sea, Northern Iran by Focusing on Echinococcus granulosus Sensu Stricto Complex

Abolghasem  SIYADATPANAH; Ahmad DARYANI; Shahabeddin SARVI; Adel SPOTIN; Mehdi SHARIF; Reza ESMAEELZADEH DIZAJI; Davood ANVARI; Amir  EMAMI ZEYDI; Mohammad Hasan KOHANSAL; Nelson Ivan  AGUDELO HIGUITA; Seyed Abdollah HOSSEINI; Mojgan ARYAEIPOUR; Shirzad GHOLAMI

doi:10.18502/ijph.v49i9.4097

Abolghasem SIYADATPANAH
Ahmad DARYANI
Shahabeddin SARVI
Adel SPOTIN
Mehdi SHARIF
Reza ESMAEELZADEH DIZAJI
Davood ANVARI
Amir EMAMI ZEYDI
Mohammad Hasan KOHANSAL
Nelson Ivan AGUDELO HIGUITA
Seyed Abdollah HOSSEINI
Mojgan ARYAEIPOUR
Shirzad GHOLAMI

DOI: https://doi.org/10.18502/ijph.v49i9.4097

Keywords: man hydatidosis; Genetic diversity; Phylogeography; Iran

Abstract

Background: Human Echinococcosisis a cyclo-zoonotic infection caused by tapeworms of the Echinococcus granulosus sensu stricto complex. The detection of mitochondrial genome data of genus Echinococcus can reflect the taxonomic status, genetic diversity, and population structure genetics.

Methods: Totally, 52 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients with histologically confirmed CE were collected from Mazandaran province, Iran in the period of Mar 1995 to May 2018. All extracted DNAs from (FFPE) tissue samples were subjected to amplify by polymerase chain reactions method targeting cytochrome c oxidase subunit 1 (cox1) gene. All PCR amplicons were sequenced to phylogenetic analysis and genetic diversity.

Results: Molecular analysis showed that 50(96.1%) and 2 (3.84%) isolates were identified as G1 andG3 E. granulosus genotypes, respectively. DNA sequence analyses indicated a high gene diversity for G1 (Haplotype diversity: 0.830) and G3 genotypes (Hd: 1.00). Based on multiple sequence alignment analyses, 7 (13.46%; G1 genotype) and 2 (3.84%; G3 genotype) new haplotypes were unequivocally identified.

Conclusion: G3 genotype (Buffalo strain) was identified from two human hydatidosis isolates in the region. Present study strengthens our knowledge about taxonomic status, transmission patterns of Echinococcus parasite to human and heterogeneity aspects of this parasite in clinical CE isolates of Northern Iran.