Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells

Yizhuo LU; Lianghui LI; Guoyang  WU; Huiqin ZHUO; Guoyan LIU; Jianchun CAI

doi:10.18502/ijph.v48i12.3551

Yizhuo LU
Lianghui LI
Guoyang WU
Huiqin ZHUO
Guoyan LIU
Jianchun CAI

DOI: https://doi.org/10.18502/ijph.v48i12.3551

Keywords: Gastric cancer; LY294002; MK-2206; Phosphorylation; PI3K/Akt signaling pathway; PRAS40- Thr246

Abstract

Background: We aimed to investigate the effect of PI3K/Akt signaling pathway on PRAS40Thr246 phosphorylation in gastric cancer cells.

Methods: The study was conducted from April 2017 to January 2018 in Zhongshan Hospital, Xiamen University, Xiamen, China. Gastric cancer cells were divided into three groups: gastric cancer cell group, LY294002 group and MK-2206 group. Specific tests were conducted accordingly.

Results: Inhibition of PI3K/Akt signaling pathway activation and PRAS40Thr246 phosphorylation could inhibit proliferation and invasion and promote apoptosis of gastric cancer cells, and PRAS40Thr246 phosphorylation could activate PI3K/Akt signaling pathway.

Conclusion: The levels of PI3K/Akt signaling pathway related proteins and p-PRAS40Thr246 were significantly increased in gastric cancer cells. p-PRAS40-Thr246 was able to reflect the activation of the PI3K/Akt signaling pathway, reflecting the sensitivity of the PI3K/AKT signaling pathway to inhibitors.