Identification of CD8+ Immunogenic Peptides for Vaccine  Design against Nipah Virus in Humans

Pankaj  Suden; Inderpal  Singh; Chitrakshi  Chopra; Malvika  Mehta; Ratna  Chandra; Indu  Bhushan

doi:10.18502/ijph.v53i12.17331

Pankaj Suden School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India
Inderpal Singh School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India
Chitrakshi Chopra School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India
Malvika Mehta School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India
Ratna Chandra School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India
Indu Bhushan School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India

DOI: https://doi.org/10.18502/ijph.v53i12.17331

Keywords: Zoonotic virus; Nipah virus; Vaccine design; Immunogenicpeptides; Molecular dynamics

Abstract

Background: Nipah virus is a pathogenic virus of ruinous zoonotic potential with inflated rate of mortality in humans.

Methods: Considering the emerging threat of this pandemic virus, the present investigation amid to design vaccine by using the bioinformatics tools such as host and virus codon usage analysis, CD8+ peptide prediction, immunogenicity/allergenicity/toxicity, MHC-I allele binding prediction and subsequent population coverage and MHC-I-peptide docking analysis.

Results: In this study (conducted in 2022 at School of Biotechnology, Katra, India), a set of 11 peptides of the structural proteins of Nipah Virus were predicted and recognized by the set of MHC-I alleles that are expressed in 92% of the global human population.

Conclusion: The strong interactions between these peptides and the MHC-I protein suggest them as strong peptide candidates for the development of vaccine against Nipah Virus.