Evaluation of Screening Results for Vancomycin-Resistant  Enterococci: Three-Year Surveillance

Zeynep  Ayaydın; Ayşe Batgi  Azarkan; Özge Alkan  Bilik; Ali Cem Tekin

doi:10.18502/ijph.v53i8.16279

Zeynep Ayaydın Department of Medical Microbiology, Diyarbakır Gazi Yaşargil Training and Research Hospital, Health Sciences University, Diyarbakır, Turkey
Ayşe Batgi Azarkan Department of Medical Microbiology, Diyarbakır Gazi Yaşargil Training and Research Hospital, Health Sciences University, Diyarbakır, Turkey
Özge Alkan Bilik Department of Medical Microbiology, Selahaddin Eyyubi State Hospital, Diyarbakır, Turkey
Ali Cem Tekin Department of Medical Microbiology, Diyarbakır Gazi Yaşargil Training and Research Hospital, Health Sciences University, Diyarbakır, Turkey

DOI: https://doi.org/10.18502/ijph.v53i8.16279

Keywords: Vancomycin-resistant enterococci; Vancomycin-resistant enterococci (VRE); Rectal swab; Real-time PCR

Abstract

Background: Enterococci are facultative anaerobic, binary, or chained Gram-positive cocci. The gastrointestinal colonization of hospitalized patients is the most important reservoir of vancomycin-resistant enterococci. We aimed to evaluate retrospectively the screening results of vancomycin-resistant enterococci, studied by the simultaneous (real-time) polymerase chain reaction method on rectal swabs of adult and pediatric patients hospitalized in our hospital in 2019-2021.

Methods: Adult and pediatric patients were included in our study between Jan 2019 and Dec 2021. The results of vancomycin-resistant enterococci, studied with the real-time polymerase chain reaction method from rectal swabs sent from intensive care units and services, were analyzed retrospectively. Isolation of the samples was performed using the Fluorion VRE QLP 1.0 real-time polymerase chain reaction kit (Iontek, Turkey), and detection was performed with the Fluorion Detection System (Iontek, Turkey) real-time polymerase chain reaction device.

Results: Overall, 31,725 patients were included in our study. When evaluated in order of years, in 2019, 379 (7%) of 5,389 adults, 322 (7.4%) of 4,003 children, 234 (5.5%) of 4,185 adults in 2020, 157 (2.4%) of 6,499 children, and in 2021, vancomycin-resistant enterococci were detected in 469 (7.5%) of 6,232 adults and 224 (4.1%) of 5,417 children.

Conclusion: The prevalence of vancomycin-resistant enterococci is greater in adults, particularly in intensive care units, compared to children. Infection control precautions and training be augmented in high-risk clinics, while the unnecessary utilization of glycopeptides should be limited.