Linoleic Acid Modulates the Expression of Metastatic and  Angiogenic Markers MMP-2 and Talin-2 in Gastric Cancer Cell Line MKN-45

Daniel  Elieh-Ali-Komi; Tohid   Kazemi; Najibeh  Shekari; Parviz  Farzamifar; Elham  Eghbali; Behzad  Mansoori; Behzad  Baradaran; Masoud  Shirmohamadi

doi:10.18502/ijph.v53i7.16055

Daniel Elieh-Ali-Komi Institute of Allergology, Charité, Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany
Tohid Kazemi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Najibeh Shekari Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Parviz Farzamifar Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Elham Eghbali Medical Radiation Sciences Research Group, Tabriz University of Medical Sciences, Tabriz, Iran
Behzad Mansoori Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Behzad Baradaran Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Masoud Shirmohamadi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

DOI: https://doi.org/10.18502/ijph.v53i7.16055

Keywords: Gastric cancer; Linoleic acid; Talin-2

Abstract

Background: Linoleic acid (LA) has modulatory effects on gastric cancer cell lines. This study aimed to investigate the effects of linoleic acid on the expression of metastatic and angiogenic molecular markers in gastric cancer cell line MKN-45.

Methods: In this study performed in Tabriz, Iran in 2021, MKN-45 cells were treated with LA in the presence or absence of docetaxel. Total RNA was extracted, and cDNA synthesized from the cells before and after treatment. The expression levels of Talin-2 and MMP-2 genes and mir-20, mir-30, mir-126, and mir-194, were determined by quantitative real-time PCR.

Results: LA treatment reduced the expression levels of mir-126, mir-194, mir-30, and MMP-2, while increased the expression levels of Talin-2 mRNA. Docetaxel treatment could decrease the expression levels of mir-20, Talin-2, and MMP-2 mRNA levels while increasing the expression levels of mir-126, mir-194, and mir-30. Additionally, the combined treatment of MKN-45 cells with LA and docetaxel could reduce the expression levels of mir-20 and mir-126 and increased the expression levels of mir-194, mir-30, Talin-2, and MMP-2 mRNAs.

Conclusion: Modulation of the expression levels of gastric cancer involved microRNAs, Talin-2, and MMP-2 may be a mechanism through which LA may exert its biological effects on GC cell line MKN-45. LA may have an antimetastatic effect by reducing the MMP-2 expression and pro-angiogenic effect through increasing Talin-2 expression levels.