Development of a Green Single Drop Microextraction Based on Deep Eutectic Solvent and HPLC-UV for Trace Residue Analysis of Three Frequent-Used Pesticides

Mehran  Pourhossein; Monireh  Khadem; Fariborz  Omidi; Omid Reza  Heravizadeh; Seyed Jamaleddin  Shahtaheri

doi:10.18502/ijph.v52i11.14043

Mehran Pourhossein Department of Occupational Health Engineering, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran
Monireh Khadem Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Fariborz Omidi Research Center for Environmental Determinants of Health, Health Institute, Kermanshah University of Medical Sciences, Kerman-shah, Iran
Omid Reza Heravizadeh Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan
Seyed Jamaleddin Shahtaheri Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/ijph.v52i11.14043

Keywords: Single drop microextraction; Metribuzin; Dichlorvos; Fenthion

Abstract

Background: A green sample preparation method named deep eutectic solvent-based single drop microextraction (DES-SDME) was developed and optimized for determining trace metribuzin, dichlorvos, and fenthion.

Methods: Two hundred seventy experimental runs were performed, and the optimal values of the five influential factors in the DES-SDME method were determined. The design of the study was based on one factor at a time and the peak area of high-performance liquid chromatography was used as a benchmark for comparing analysis results.

Results: After optimizing the effective factors, the linearity range, detection limit and quantification limit of the method were determined by drawing calibration curves for the studied analytes.

Conclusion: The results indicated the success of the developed method in obtaining acceptable figures of merit as a green preparation method with accuracy and precision.