Overexpression of S100A1 in Osteosarcoma Inhibits Tumor  Proliferation and Progression

Wenhua  Xing; Yanbin  Zhao; Liuwan  Lin; Zhenqun  Zhao; Mengchen  Yang; Na Wang; Shuxia  Cui; Rui  Bai; Aiqing  Zhao

doi:10.18502/ijph.v51i12.11468

Wenhua Xing The Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot, Inner Mongolia, 10030, China
Yanbin Zhao Health Care Center of Ning Bo Customs, Ningbo City, Zhejiang Province, 315012, China
Liuwan Lin The Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot, Inner Mongolia, 10030, China
Zhenqun Zhao The Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot, Inner Mongolia, 10030, China
Mengchen Yang The Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot, Inner Mongolia, 10030, China
Na Wang The Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot, Inner Mongolia, 10030, China
Shuxia Cui The Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot, Inner Mongolia, 10030, China
Rui Bai The Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot, Inner Mongolia, 10030, China
Aiqing Zhao Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, 10030, China

DOI: https://doi.org/10.18502/ijph.v51i12.11468

Keywords: S100A1 protein; Osteosarcoma; Proliferation; Progression

Abstract

Background: Osteosarcoma is the most common primary malignant tumor of bone. Abnormal expression of S100A1 protein is closely related to the occurrence and development of malignant tumors. However, S100A1 in osteosarcoma has not been studied.

Methods: All osteosarcoma tissues were collected from patients who received surgical therapy at the Affiliated Hospital of Inner Mongolia Medical University, China in 2020. QRT-PCR and western blot assays were used to detect the expression of S100A1 in osteosarcoma tissues and cells. The negative effect of S100A1 on osteosarcoma cell growth was confirmed by vitro and vivo experiments.

Results: S100A1 inhibited the growth of osteosarcoma cells in vitro. Overexpression of S100A1 may inhibit the proliferation of osteosarcoma cells by preventing the activation of AKT signaling pathway by western blot assay. Finally, animal experiments confirmed that overexpression of S100A1 could inhibit the proliferation of osteosarcoma cells. Overexpression of S100A1 obtained better survival benefit in mice.

Conclusion: Our findings provided a new insight to the treatment of osteosarcoma. It also raised the possibility that S100A1 could be used in targeted therapies for osteosarcoma.