Assessment of Non-Coding RNAs (miR-506 and circRNA 000284) and their Target Gene SNAIL-2 in Breast Tumors: Implications for Prognosis and a Possible Circulating Biomarker

Shamim Dashti  Gohari; Mahdieh  Salimi

doi:10.18502/bccr.v15i2.18732

Shamim Dashti Gohari Department of Medical Genetics, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Mahdieh Salimi Department of Medical Genetics, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

DOI: https://doi.org/10.18502/bccr.v15i2.18732

Keywords: breast cancer, circRNA000284, miR-506, noncoding RNAs, prog- nostic biomarker

Abstract

Background: Breast cancer is the most common malignancy among women, andearly diagnosis and targeted therapy have garnered significant attention. Non-cod-ing RNAs have emerged as potential diagnostic, prognostic, and treatment biomark-ers for breast cancer. This study aimed to evaluate the expression of non-codingRNAs, specifically miR-506 and circular RNA 000284, and their target gene SNAIL-2in breast cancer patients compared to normal controls. The study also focused onclinicopathological characteristics, and plasma was monitored for expression ofcirc0000284 to identify a possible accessible cancer-related marker.

Methods: Using the SYBR-Green Real-time PCR technique, circ0000284, miR-506,and SNAIL2 expression were analyzed in total RNA extracted from 80 breast tumorscompared with normal control. Also, the expression of circ0000284 was monitored inthe plasma of breast cancer patients. The association of circ0000284, miR-506, andSNAIL2 expression with clinicopathological characteristics were studied.

Results: The results showed overexpression, down-regulation, and up-regulationof circRNA 000284, miR-506, and SNAIL-2, respectively. These expression changeswere associated with advanced stages of the disease and lymph nodal involvement,which are signs of a poor prognosis (<0. 0001). Additionally, a positive direct cor-relation was observed between circRNA000284 expression in tumors and plasma(<0. 0001). Moreover, it was discovered that circ-0000284 sponged miR-506, causingup-regulation of SNAIL-2 as its mRNA target.

Conclusion: The upregulation of SNAIL-2 as an epithelial-mesenchymal-transi-tion (EMT) factor leads to poor prognosis in breast cancer and is epigeneticallyregulated by miR-506 and circRNA 000284. Therefore, the overexpression of circR -NA000284 in plasma could be considered an indicator of lymph nodal involvementand advanced stages of cancer, and nominated as a poor prognostic biomarker forfuture considerations