Comparing the Immunity of Genetically and Non-Genetically Modified Rice regarding the Possibility of Gene Transferring to Blood of a Laboratory Animal

  • Bahador Haji Mohammadi
  • Gilda EIslami
  • Mahsa Aalaei
  • Mohammad Hassan EhramPoush
  • Mohammad Ebrahim Rezvani
  • Hossein Fallahzadeh
  • Mehrnooush Shirdeli
Keywords: Transgenic Rice, Rat Sprague Dawley, Blood, Cry1A (b), P35

Abstract

Introduction: Genetically modified plant species are produced by the genetic engineering of agricultural science and the addition of specific genes in their genetic sequences with the aim of optimizing the product and creating desirable traits. This study examined the remains of Cry1A(b) and P35 genes in blood of rats fed with genetically modified rice.

Methods: This experimental and interventional study was conducted on male and female rats Sprague Dawley (SD), which were divided into treatment and control groups according to the type of food received. Each group consisted of 10 rats (5 male and 5 female); the first group consumed 50 percent of the genetically modified rice and the second group consumed 50percent of non- genetically modified rice from the total needed carbohydrate. Blood sampling and DNA extraction were conducted after 90 days of feeding rats with nutritional pattern using a kit. Quantitative and qualitative tests were carried out on the extracted DNA using agarose gel electrophoresis and spectrophotometry, respectively.

Results: Agarose gel electrophoresis was used and the results showed no transgenic genes in the studied blood samples.

Conclusion: The results showed no significant difference between the the treatment and control rats with regard to the presence of transgenic genes of p35 and Cry1A(b). Therefore, the possibility of gene transfer to the organs of rats consuming genetically modified rice was rejected. Results of this study showed no difference between the safety of genetically-modified and non- modified rice with regard to gen transferring.

Published
2019-05-13
Section
Articles