Genetic Factors of Idiopathic Recurrent Miscarriage in Kazakh Population

Gulnara S. Svyatova; Dinara D.  Mirzakhmetova; Galina M. Berezina; Alexandra V. Murtazaliyeva

doi:10.18502/jri.v23i1.8451

Gulnara S. Svyatova Scientific Center of Obstetrics, Gynecology and Perinatology, Almaty, Republic of Kazakhstan
Dinara D. Mirzakhmetova Scientific Center of Obstetrics, Gynecology and Perinatology, Almaty, Republic of Kazakhstan
Galina M. Berezina Scientific Center of Obstetrics, Gynecology and Perinatology, Almaty, Republic of Kazakhstan
Alexandra V. Murtazaliyeva Scientific Center of Obstetrics, Gynecology and Perinatology, Almaty, Republic of Kazakhstan

DOI: https://doi.org/10.18502/jri.v23i1.8451

Keywords: Gene polymorphism, Genotypes, Implantation, Pregnancy, Reproductive medicine.

Abstract

Background: It seems that 50% of the possible causes of recurrent miscarriage do not have any explainable etiology and they require in-depth etiopathogenesis analysis. The purpose of this research was to study polymorphisms relationship of the im-mune response genes including Val249Ile CX3CR1 (rs3732379), CT60 G/A CTLA4 (rs3087243), and HLA DQA1, DQB1, DRB1 (major histocompatibility complex, class II) with development of idiopathic form of recurrent miscarriage (iRM) in Kazakh population.

Methods: TagMan genotyping for 302 patients with iRM and 300 women with normal reproduction was performed. Molecular genetic studies were carried out by the TaqMan method of unified site-specific amplification and real-time genotyping using test systems. Statistical tests and Chi Square were carried out using PLINK, STATA13 software and p˂0.05 was considered statistically significant.

Results: It has been shown that carriage of unfavorable genotypes (Val/Ile, Val/Val) by the Val249Ile polymorphism of CX3CR1 gene increases the risk of developing iRM by 1.43 times. Search for associations of genes allelic variants of HLA class 2 complex with iRM revealed 501 allele in DQA1 locus, 0301 in DQB1 locus, 10, 12, 15, 16 alleles in DRB1 locus, which increase the risk of developing iRM in Kazakh population.

Conclusion: The highly significant associations of immune response genes with development of iRM in Kazakh population indicate the possible involvement of the immune system interaction of mother cells with syncytiotrophoblast, which is realized by vascular defects and defective embryo implantation, causing termination of pregnancy.