Serum Levels of CCN3 Protein in Iranian Women with Polycystic Ovary  Syndrome

Akram  Vatannejad; Fatima Zahraa  Fouani; Reza  Fadaei; Asma  Kheirollahi; Soheila  Ansaripour; Maryam Shabani  Nashtaei; Nariman  Moradi; Hossein  Montakhab-Yeganeh; Faezeh  Fattahi; Farah  Jadidizadeh

doi:10.18502/jri.v26i2.19435

Akram Vatannejad Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran,
Fatima Zahraa Fouani Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medi-cal Sciences, Tehran, Iran
Reza Fadaei Sleep Disorders Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Asma Kheirollahi Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Soheila Ansaripour Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Maryam Shabani Nashtaei Department of Infertility, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
Nariman Moradi Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
Hossein Montakhab-Yeganeh Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Faezeh Fattahi Emergency Department, Shahid Fayazbakhsh Hospital, Tehran, Iran
Farah Jadidizadeh Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

DOI: https://doi.org/10.18502/jri.v26i2.19435

Keywords: Abortion, C-reactive protein, CCN3, Homocysteine, Metabolic syndrome, Nephroblastoma overexpressed protein, Polycystic ovary syndrome.

Abstract

Background: Polycystic ovary syndrome (PCOS) is a heterogeneous condition that encompasses several cardiometabolic and endocrinological disorders. Studies have shown that its pathogenesis aligns with several underlying mechanisms associated with recurrent pregnancy loss (RPL). Cellular communication network (CCN)-3 protein is a well-studied adipokine involved in tumorigenesis, organogenesis, inflammation, fibrosis, and glucose metabolism. The purpose of the current study was to determine the association of CCN3 levels with a number of parameters involved in PCOS pathogenesis.

Methods: This is a case-control study including 120 PCOS patients (60 cases with RPL; PCOS-RPL and 60 cases with infertility; PCOS-Inf and 60 healthy controls). Circulating levels of homocysteine and high-sensitivity C-reactive protein (hs-CRP), homocysteine, and CCN3 were measured using ELISA kits.

Results: Circulating levels of CCN3 were significantly elevated in PCOS-RPL and PCOS-Inf subgroups when compared to the control group (7.61±3.03 and 6.85±2.54 vs. 3.12±0.82, p<0.001). Serum CCN3 positively correlated with fasting insulin and homeostatic model assessment of insulin resistance (HOMA-IR) in the control group (p<0.05) and PCOS group (p<0.001). Moreover, CCN3 was significantly associated with PCOS (OR 4.808, 95%CI [2.744-8.423], p<0.001).

Conclusion: According the results of this study CCN3 may be involved in the pathogenesis of PCOS. However, future studies are needed to evaluate the possibility of utilizing CCN3 in the diagnosis and treatment of the disease.