The Impact of Endometriosis on Intracellular Calcium Levels, Cyclic  Dependent Kinase 1 (Cdk1) Expression, and Cyclin B Expression in Post-Ovulation Oocytes of Mice Model

Teguh  Wiyono; Sri Ratna  Dwiningsih; Widjiati  Widjiati

doi:10.18502/jri.v24i4.14150

Teguh Wiyono Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia
Sri Ratna Dwiningsih Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia
Widjiati Widjiati Department of Veterinary Embryology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia

DOI: https://doi.org/10.18502/jri.v24i4.14150

Keywords: Calcium, Cdk1, Cyclin B, Endometriosis, Oocyte

Abstract

Background: Since endometriosis causes a decrease in oocyte quality, the success rate of in vitro fertilization cycles decreases. The purpose of the current study was to analyze the effect of endometriosis on intracellular calcium levels, Cdk1 expression, and cyclin B expression in oocytes.

Methods: Thirty-two mice (Mus musculus) were divided into control and endo-metriosis groups. The cumulus oocyte complex (COC) were obtained in all groups. Denudated cells were assessed for calcium levels by calorimetric examinations. Complex oocytes were examined for Cdk1 and cyclin B expression by immune-cytochemistry and were read under a microscope.

Results: Intercellular calcium levels, Cdk1, and cyclin B expression were signifi-cantly lower in the endometriosis group than in the control group. There was a signi-ficant relationship between calcium levels and Cdk1 expression (p<0.05, r=0.659), a significant relationship between calcium levels and cyclin B expression (p<0.05, r=0.885), and also a significant correlation between Cdk1 and cyclin B expression (p<0.05, r=0.537).

Conclusion: The data presented in this study suggested that the intracellular oocyte calcium level, Cdk1 expression, and cyclin B expression were lower in mice with endometriosis. A positive correlation was observed between calcium levels and the expression of Cdk1 and cyclin B. Furthermore, a positive correlation was also found between Cdk1 and cyclin B expression.