A Comparison Between the Effect of Low-Level Laser Therapy and Clomiphene on Rats’ Ovarian Tissue Blood Vessels Under In Vivo Conditions

Paria Naseri; Seyed Hosein Rasta; Aliraza Alihemati

doi:10.18502/jimc.v5i2.10470

Paria Naseri Department of Medical Physics, Tabriz University of Medical Sciences, Tabriz, Iran
Seyed Hosein Rasta Department of Medical Physics, Tabriz University of Medical Sciences, Tabriz, Iran
Aliraza Alihemati Stem Cell Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran

DOI: https://doi.org/10.18502/jimc.v5i2.10470

Keywords: Animals, Clomiphene, Female, Infertility, Lasers, Ovary, Prevalence

Abstract

Background: Studying the prevalence of infertility as a common disease and its treatment have recently become an important issue. These treatments include drugs and surgery; and lasers have also been used complementarily. However, they affect the ovarian blood vessels number. Accordingly, the present study was conducted to investigate the photo-bio stimulation effect on near-infrared and red laser as of low-level lasers on the blood vessels in ovarian tissue, compared with the clomiphene’s effect.

Methods: Twenty-eight female rats were randomly divided into four groups: Control (CT), Clomiphene Drug (D), Red Laser (RL) and Near-Infrared Laser (NIRL). Afterwards, the laser groups received irradiation (5 J/cm² dose) and the rats in group D received clomiphene (1µg/kg). After the experiment, the animals were anesthetized and their ovaries were removed. Henceforth, the laser’s effect was investigated and compared with that of clomiphene.

Results: The number of the blood vessels increased in NIRL (71.96%) and RL (67.070%) groups, compared with CT. It was also increased in NIRL (59.81%) and RL (14%) compared to D group. In addition, it increased in NIRL (53.27%) compared with RL. This increase was significant in the NIRL group indicating that NIRL increases ovarian activity to produce blood vessels that can be certainly used in future studies for finding a cure for ovarian negligence to produce more blood vessels and treat diseases caused by it.

Conclusion: The results convinced us to introduce laser as a new method to increase the ovarian activation by increasing the blood vessels in a future study. Although the mechanism of low-level lasers action is not well known, it has been proved to be more effective than clomiphene.