Development of a Novel Antimicrobial Active Surface via Integration of tetracycline within an Acrylate Coating and Soluble Soybean Polysaccharide Matrix

Authors

  • D. Salarbashi Department of Nutrition, Food Science and Clinical Biochemistry, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
  • M. Tafaghodi Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
  • S.A. Mohajeri Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
  • B. Jafari Sustainable Membrane Technology Research Group, Department of Chemical Engineering, Faculty of Petroleum, Gas and Petrochemical Engineering, Persian Gulf University, 75169, Bushehr, Iran
  • M. Saghravanian Department of Chemistry, Mashhad Branch, Islamic Azad University, Mashhad, Iran
  • F. Hadizadeh Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
  • B.S. Fazly Bazzaz Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
  • L. Ansari Cellular and molecular research center, cellular and molecular medicine research institute, Urmia university of medical sciences, Urmia, Iran
  • S. Tafazzoli Mehrjardi Pharmaceutics Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • Jamal Al- Sabahi Central Instrumentation Laboratory, College of Agricultural and Marine Sciences, Sultan Qaboos University, Oman
  • U.H. Al Hoqani Applied Sciences Department, College of Applied Sciences and Pharmacy, University of Technology and Applied Sciences, Muscat, Oman

DOI:

https://doi.org/10.18502/jfqhc.13.2.22140

Keywords:

Decontamination, Plasma Gases, Anti-Bacterial Agents, Tetracycline, Surface Properties

Abstract

Background: The proliferation of harmful germs on surfaces necessitates the development of active, self-decontaminating materials. This study aims to develop a novel antimicrobial active surface.

Methods: A double-layer bionanocomposite was fabricated. The base layer was a film of Soluble Soybean Polysaccharide (SSPS) and nanoclay Na⁺. Using Molecularly Imprinted Polymer (MIP) technology, tetracycline was integrated into an acrylate coating, which was then applied to the polysaccharide film via oxygen plasma treatment to form the active top layer. The composite was characterized using Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and dynamic mechanical analysis. Its antibacterial activity against Staphylococcus aureus was evaluated through a time-kill study over 24 h. All experiments were performed in triplicate (n=3). Statistical analysis was conducted using ANOVA followed by Duncan's multiple range test

Results: Spectroscopy confirmed the integration of components. Morphological analyses showed a uniform, defect-free bilayer structure with strong interfacial adhesion. The incorporation of nanoclay significantly improved the mechanical properties, increasing the storage modulus and Glass Transition Temperature (Tg). Time-kill assays demonstrated a sustained release of tetracycline, leading to a bacteriostatic effect and a complete eradication of S. aureus after 24 h.

Conclusion: The developed bilayer composite successfully functions as an active surface with prolonged self-decontaminating properties, achieved through the sustained release of tetracycline. This promising material can inhibit microbial growth on surfaces, potentially extending the shelf-life of products.

Published

2026-07-28

Issue

Volume (13) issue (2)

Section

Articles