Gamma Irradiation of Brown Rice: Effects of Dose, Variety, Paddy Age, and Packaging on Fungal Control, Shelf-Life and Post-Harvest Cost Estimate

  • G.B. Abrera Biomedical Research Section, Department of Science and Technology – Philippine Nuclear Research Institute (DOST-PNRI), Commonwealth Avenue, Diliman, Quezon City, Philippines
  • G. Diano Biomedical Research Section, Department of Science and Technology – Philippine Nuclear Research Institute (DOST-PNRI), Commonwealth Avenue, Diliman, Quezon City, Philippines
  • M.M. Tolentino Biomedical Research Section, Department of Science and Technology – Philippine Nuclear Research Institute (DOST-PNRI), Commonwealth Avenue, Diliman, Quezon City, Philippines
  • M.C.B. Gragasin Philippine Center for Postharvest Development and Mechanization, Science City of Munoz, Nueva Ecija, Philippines
  • M.L.C. Cobar Biomedical Research Section, Department of Science and Technology – Philippine Nuclear Research Institute (DOST-PNRI), Commonwealth Avenue, Diliman, Quezon City, Philippines
  • G. Caraos Biomedical Research Section, Department of Science and Technology – Philippine Nuclear Research Institute (DOST-PNRI), Commonwealth Avenue, Diliman, Quezon City, Philippines
  • Z.M. De Guzman Biomedical Research Section, Department of Science and Technology – Philippine Nuclear Research Institute (DOST-PNRI), Commonwealth Avenue, Diliman, Quezon City, Philippines
  • H.M. Solomon Irradiation Services Section, Department of Science and Technology – Philippine Nuclear Research Institute (DOST-PNRI), Commonwealth Avenue, Diliman, Quezon City, Philippines
  • C.O. Asaad Biomedical Research Section, Department of Science and Technology – Philippine Nuclear Research Institute (DOST-PNRI), Commonwealth Avenue, Diliman, Quezon City, Philippines
  • C.C. Deocaris Biomedical Research Section, Department of Science and Technology – Philippine Nuclear Research Institute (DOST-PNRI), Commonwealth Avenue, Diliman, Quezon City, Philippines
Keywords: Aspergillus niger, Food Contamination, Food Irradiation, Food Packaging, Radiation, Gamma

Abstract

Background: Brown rice offers superior nutritional value compared to polished white rice but is less favored in rice-consuming countries due to its limited shelf-life. This study evaluates the efficacy of gamma irradiation in reducing fungal contamination in brown rice and extending its shelf-life, considering factors such as paddy age, rice variety, and packaging type.

Methods: The efficacy of gamma irradiation (0-1 kGy) in reducing fungal contamination in brown rice was evaluated. RC-160 and SL-7 rice varieties (240 kg per variety) were harvested from Central Luzon (Region III) during the dry season (March–April 2023). One kg of two and eight-week-old paddy grains were packed in either Super Bag™, an International Rice Research Institute developed hermetic packaging, or conventional Polyethylene bags. Mold and Yeast Counts were quantified using 3M Petrifilm™ following Association of Official Agricultural Chemists official methods. Fifty g of each sample were homogenized in Butterfield’s phosphate buffer, serially diluted, plated, and incubated at 25±1 °C for 3-5 days. Statistical analyses were performed using one-way ANOVA and Tukey’s HSD post-hoc test with Statistical Tool for Agricultural Research (STAR) software, version 2.0.1 (IRRI). 

Results: Gamma irradiation at one kGy significantly (p<0.05) reduced Mold and Yeast Counts, with effects sustained for up to eight months. Paddy age and packaging type had no significant influence on fungal load. A dose of 0.58 kGy achieved a 1-log (90%) reduction in Aspergillus niger, while 3 kGy achieved complete inactivation, extending shelf-life up to six months. Cost analysis showed irradiation, logistics, and added storage fees increased retail price per kg by 0.14, 0.04, and US$0.02, respectively. 

Conclusions: Gamma irradiation effectively controls fungal contamination and extends brown rice shelf-life, potentially enhancing consumer confidence and promoting wider adoption of brown rice. 

Published
2025-06-09
Section
Articles