Evaluating the blood toxicity of functionalized graphene-arginine with anticancer drug ginsenoside Rh2 in balb/c mouse model with breast cancer

Shervin Dokht Farhangfar; Farzaneh Fesahat; Sayed Mohsen Miresmaeili; Hadi Zare-Zardini

doi:10.18502/ijpho.v12i1.8356

Shervin Dokht Farhangfar Department of Biology, Science and Arts University, Yazd, Iran
Farzaneh Fesahat Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Sayed Mohsen Miresmaeili Department of Biology, Science and Arts University, Yazd, Iran
Hadi Zare-Zardini Hematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

DOI: https://doi.org/10.18502/ijpho.v12i1.8356

Keywords: Gensenoside Rh2, balb/c mouse, Graphene-Arginine, Graphene-Arginine-ginsenoside Rh2, blood

Abstract

Background: Gensenoside Rh2 is an anticancer drug with low toxicity and stability in the body. The aim of this study was to evaluate the blood toxicity of functionalized graphene-arginine with anticancer drug ginsenoside Rh2 in balb/c mouse model with breast cancer.

Materials and Methods: Graphene-Arginine (G-Arg) and Graphene-Arginine-ginsenoside Rh2 (G-Arg-Rh2) were synthesized using microwave method. For evaluation of blood toxicity, 32 mice with breast tumors were randomly divided into 4 groups: control (3mg/kg 6 mg / kg PBS sterile), group 1 (6 mg / kg ginsenoside), group 2 (3 mg / kg G-Arg), and group 3 (3 mg / kg G-Arg-Rh2). Treatment was done intravenously once every three days for 32 days. Finally, blood factors were also examined by sampling from the heart.

Results: Complete functionalization was proven by FTIR and Raman. Examination of blood factors showed that white blood cells had a very small increase. Anova test showed significant difference among four groups in term of WBC count (p=0.016). Pair sample T test showed that there was significant difference between control and group 1(p=0.036) and control and group 2 (p=0.036). There was no significant difference between control and group 3 (p=0.051). Other blood factors had no significant difference among examined groups (p>0.05).

Conclusion: Based on results, after treatment with all designed nanostructures, only white blood cells had a very small increase and inflammatory reactions were statistically similar in all groups. This indicates the high efficiency of designed drug.