Molecular characterization of Vibrio cholerae O1 strains circulating in Assam: a north eastern state of India

Ajanta Sharma; Bornali Sarmah Dutta; Debajit Rabha; Elmy Samsun Rasul; Naba Kumar Hazarika

doi:10.18502/ijm.v13i5.7420

Ajanta Sharma Department of Microbiology, Gauhati Medical College, Guwahati, Assam
Bornali Sarmah Dutta Department of Microbiology, Gauhati Medical College, Guwahati, Assam
Debajit Rabha Department of Microbiology, Gauhati Medical College, Guwahati, Assam
Elmy Samsun Rasul Department of Microbiology, Fakaruddin Ali Ahmed Medical College, Barpeta, Assam
Naba Kumar Hazarika Department of Microbiology, Gauhati Medical College, Guwahati, Assam

DOI: https://doi.org/10.18502/ijm.v13i5.7420

Keywords: Vibrio cholerae O1; Cholera toxin; Virulence; Genotype; Drug resistance

Abstract

Background and Objectives: Information on the genetic epidemiology of cholera in Assam, a northeastern state of India is lacking despite cholera being a major public health problem. The study aimed to determine the virulence genes and genes encoding antibiotic resistance in Vibrio cholerae isolates and to determine the prevalent genotypes based on the presence or absence of the virulence genes and ctxB genotype.

Materials and Methods: Twenty-five V. cholerae strains were subjected to conventional biotyping and serotyping followed by multiplex PCR to detect ctxA, ctxB, zot, ace, O1rfb, tcpA, ompU, ompW, rtxC, hly and toxR and antibiotic resistance genes. Cholera toxin B (ctxB) gene was amplified followed by sequencing.

Results: All the V. cholerae O1 isolates were El Tor Ogawa and showed the presence of the core toxin region representing the genome of the filamentous bacteriophage CTXø. The complete cassette of virulence genes was seen in 48% of the isolates which was the predominant genotype. All the isolates possessed amino acid sequences identical to the El Tor ctxB subunit of genotype 3. sulII gene was detected in 68% of the isolates, dfrA1 in 88%, strB in 48% and SXT gene was detected in 36% of the isolates.

Conclusion: Toxigenic V. cholerae O1 El Tor Ogawa strains of ctxB genotype 3 carrying a large pool of virulence genes are prevailing in Assam. Presence of a transmissible genetic element SXT in 36% of the strains is of major concern as it indicates the emergence of multiple drug resistance among the V. cholerae isolates.