Development of multiplex PCR for rapid detection of metallo-β-lactamase genes in clinical isolates of Acinetobacter baumannii

Abstract

Background and Objectives: Acinetobacter baumannii has been known as a major pathogen causing nosocomial infec- tions. The aim of this study was to develop multiplex PCR for rapid and simultaneous detection of metallo-β-lactamase (MBL) genes in clinical isolates of A. baumannii.

Materials and Methods: In this study, we used three sets of primers to amplify the MBL genes including bla        ,

bla

and bla

OXA-48

. The multiplex PCR assay was optimized for rapid and simultaneous detection of MBL genes in A. bau-

OXA-23

NDM

mannii strains recovered from clinical samples.

Results: A. baumannii strains recovered from clinical samples were subjected to the study. The multiplex PCR produced 3

OXA-48

 

OXA-23

 

bands of 501 bp for bla        , 744 bp for bla

observed in multiplex PCR.

OXA-48

and 623 bp for bla

NDM

genes. In addition to, no any cross-reactivity was

Conclusion: Based on obtained data, the multiplex PCR had a good specificity without any cross reactivity and it appears

that the multiplex PCR is reliable assay for simultaneous detection of MBL genes in A. baumannii strains.