Exposure to thermal stress induces overexpression of protein-L-iso aspartate-O-methyl transferase (PIMT) in Pseudomonas aeruginosa

Authors

  • Suman Agrawalla Department of Microbiology, College of Basic Sciences and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India
  • Prasanta Kumar Koustasa Mishra Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India
  • Srinivas Sathapathy Department of Veterinary Anatomy and Histology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India
  • Ashok Kumar Sethi Department of Microbiology, College of Basic Sciences and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India
  • Debasis Dash Department of Botany, College of Basic Sciences and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India
  • Archana Mahapatra Department of Veterinary Anatomy, Faculty of Veterinary and Animal Sciences, Banaras Hindu university, Rajiv Gandhi South Campus, Uttar Pradesh, India
  • Radhakrishna Sahu Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India

DOI:

https://doi.org/10.18502/ijm.v18i4.22206

Keywords:

Protein D-aspartate-L-isoaspartate methyltransferase; Pseudomonas aeruginosa; Heat stress; Deamidation; En- zyme-linked immunosorbent assay; Blot; Western

Abstract

Background and Objectives: Apart from spontaneous deamidation and isomerization of Asp/Asn residues, factors like age- ing, oxidative stress, salinity and heat stress may also form abnormal isoaspartate (iso-Asp) residues compromising protein structure and function. PIMT helps in restoration of such iso-Asp residues. Pseudomonas aeruginosa is a gram-negative bacteria responsible for diseases with varied degree of pathogenesis in humans and animals. They can survive a wide range of temperature between 25℃ to 42℃. The objective of the current study was to monitor the expression dynamics of PIMT in Pseudomonas aeruginosa upon exposure to different thermal stresses.

Materials and Methods: The mid log phase culture of Pseudomonas aeruginosa was exposed to different temperature (37°C, 42°C, 56°C, 80°C) for an hour. The expression levels of PIMT were quantified by Western blot and ELISA.

Results: A relatively higher level of expression of PIMT was observed in the group exposed to 42°C, 56°C, 80°C in compar- ison to 37°C. The expression of PIMT increased around 1.5 times in the group exposed to 42°C.

Conclusion: A temperature dependent overexpression of PIMT was observed in Pseudomonas aeruginosa.

Published

2026-08-03

Issue

Volume (18) issue (4)

Section

Articles