Antimicrobial and antioxidant properties of green synthesis CuO nanoparticles using Salvia officinalis hydroalcoholic extract

Abdolrazagh  Marzban; Pegah  Shakib; Seyedeh Zahra  Mirzaei; Gholamreza  Goudarzi; Fatemeh  Jahani

doi:10.18502/ijm.v18i3.21660

Abdolrazagh Marzban Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Pegah Shakib Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Seyedeh Zahra Mirzaei Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences (KUMS), Kermanshah, Iran
Gholamreza Goudarzi Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Fatemeh Jahani Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

DOI: https://doi.org/10.18502/ijm.v18i3.21660

Keywords: Green synthesis; Copper oxide nanoparticles (CuO-NPs); Antimicrobial; Antioxidant; Salvia officinalis

Abstract

Background and Objectives: Following the emergence of microbial resistance to chemical antimicrobial agents, research- ers have recently focused on the effects of nanoparticles and their antimicrobial activity. This research aimed to investigate the antimicrobial and antioxidant activity of copper oxide nanoparticles (CuO-NPs) produced using the hydroalcoholic extract of Salvia officinalis (S. officinalis).

Materials and Methods: In this study, after preparing the hydroalcoholic extract of S. officinalis and synthesizing CuO-NPs using this extract, the synthesized nanoparticles were evaluated. The antimicrobial properties of synthesized CuO-NPs were investigated by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microbroth dilution method. The minimum biofilm inhibitory concentration (MBIC) and antioxidant activity of CuO-NPs and the hydroalcoholic extract of S. officinalis against Pseudomonas aeruginosa (P. aeruginosa) were investigated.

Results: CuO-NPs have a size in the range of 40 nm and a monoclinic crystal structure. Antimicrobial activity assessment by well diffusion showed that the synthesized CuO-NP nanoparticles at concentrations below 5 mg/mL had no antimicrobial effect on any of the bacteria studied. The lowest MIC of the synthesized CuO-NPs was observed against Staphylococcus saprophyticus (S. saprophyticus), and the highest against P. aeruginosa and Bacillus cereus (B. cereus). On the other hand,the highest MBC was related to B. cereus. The plant extract exhibited much weaker antimicrobial activity than CuO-NPs.50 The results obtained showed that the MBIC for CuO-NPs was 312 μg/mL. The half-maximal inhibitory concentration (IC )obtained from CuO-NPs and the hydroalcoholic extract of S. officinalis was 2.79 and 1.97mg/ml, respectively.

Conclusion: Based on the results of this study, the green synthesis of CuO-NPs using hydroalcoholic extract of S. officinalis is a suitable candidate for the preparation of antimicrobial and antibiofilm compounds that also have antioxidant properties.