Chromosome-borne blaCTX-M-65 gene in non-O1/O139 Vibrio cholerae   isolated from bile

Haifeng  Mao; Xinzhe  Wu; Danting  Jin

doi:10.18502/ijm.v18i1.20906

Haifeng Mao Department of Clinical Laboratory, The First Affiliated Hospital of Kangda College of Nanjing Medical University, The First People's Hospital of Lianyungang, Lianyungang, China
Xinzhe Wu Department of Clinical Laboratory, The First Affiliated Hospital of Kangda College of Nanjing Medical University, The First People's Hospital of Lianyungang, Lianyungang, China
Danting Jin Department of Clinical Laboratory, The First Affiliated Hospital of Kangda College of Nanjing Medical University, The First People's Hospital of Lianyungang, Lianyungang, China

DOI: https://doi.org/10.18502/ijm.v18i1.20906

Keywords: Vibrio cholerae; Bile; Sequence analysis; DNA; Drug resistance; Virulence factors

Abstract

Background and Objectives: Non-O1/O139 Vibrio cholerae (NOVC) has been associated with extraintestinal infections; however, its isolation from bile remains exceedingly rare. This study reports the identification and comprehensive analysis of a NOVC strain isolated from bile, including its antimicrobial resistance profile, virulence gene content, and molecular characteristics.

Materials and Methods: A NOVC isolate was obtained from the bile of a patient with a hepatobiliary tumor. The isolate was identified and subjected to antimicrobial susceptibility testing. Whole-genome sequencing was performed to characterize its molecular features, including antimicrobial resistance genes, virulence genes, and relevant genetic mutations.

Results: The NOVC isolate presents a multi-drug resistance phenotype. Corresponding genomic analysis indicates that thisstrain belongs to a novel sequence type (ST1736), carrying various drug resistance genes and virulence factors. Moreover,the blaCTX-M-65extended-spectrum β-lactamase (ESBL) gene was detected in its chromosomal genome.

Conclusion: This study presents the first report of a multidrug-resistant NOVC strain isolated from bile in mainland China.Notably, the ESBL gene blaCTX-M-65was identified chromosomally in NOVC for the first time.