Assessing the beneficial effect of Lactobacillus plantarum MS1 and Lactobacillus delbrueckii YN1 on colitis in a rat model

Manizheh Hajizadeh  Varzeghan; Gholamreza  Zarrini; Farzam Sheikhzadeh  Hesari; Mohammad Hossein  Modarressi

doi:10.18502/ijm.v17i5.19889

Manizheh Hajizadeh Varzeghan Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Gholamreza Zarrini Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Farzam Sheikhzadeh Hesari Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Mohammad Hossein Modarressi Department of Medical Genetics, Faculty of Medicine, Tehran University of Medicine Science, Tehran, Iran

DOI: https://doi.org/10.18502/ijm.v17i5.19889

Keywords: Inflammatory bowel disease; Probiotics; Inflammatory cytokines; Lactic acid bacteria

Abstract

Background and Objectives: Probiotics are effective in improving inflammatory bowel disease (IBD). This study assessed the effect of mesalazine and two candidate probiotics on the improvement of acetic acid (AA)-induced colitis model. Materials and Methods: Lactobacillus plantarum MS1and Lactobacillus delbrueckii YN1 were used for IBD model in rat. Twenty-five male Wistar rats weighing 250 ± 50 grams were used in 5 classified groups: Control (CO); Colitis (CL); Colitis, Probiotic (CLP); Colitis, Mesalazine (CLM); Colitis, Probiotic, Mesalazine (CLPM) and the treatment period was 3 weeks. The rats were treated with mesalazine 30 mg/kg and probiotic 109 CFU/ml after induction of colitis. Histopathological and immunological analyses were performed to evaluate the effects of probiotic bacteria on IBD.

Results: The results showed that the probiotic bacteria reduced inflammation (P<0.05), extent (P<0.01), crypt abscesses (P<0.01), edema (P<0.05), inflammatory cell infiltration (P<0.5), and increased mucosa (P<0.001) in rats. Mesalazine admin- istration in animals with colitis did not have a significant effect. Administration of probiotics in both CLP and CLPM groups reduced extent, crypt abscesses, edema, and inflammatory cell infiltration and showed an important role in the down-regu- lation of consolidation of pro-inflammatory factors (TNFα, IL-6, and IL-17), as well as up-regulation of anti-inflammatory factors such as IL-10.

Conclusion: Lactobacillus plantarum MS1 and Lactobacillus delbrueckii YN1 have shown significant potential in alleviat- ing AA-induced colitis symptoms. Their administration leads to a marked reduction in pro-inflammatory cytokines such as TNF-α, IL-17, and IL-6, while enhancing IL-10 levels, indicating their promise as therapeutic candidates for inflammatory bowel disease (IBD).