Anti-candida activity of Lacticaseibacillus rhamnosus R-2002 and its possible application in candidiasis prevention

Aleksanyan  Tigran; Hakobyan  Lusine; Harutyunyan  Ruzanna; Semerjyan  Inesa; Nahapetyan  Nelli; Gharibyan  Edita; Haertlé  Thomas; Bazukyan  Inga

doi:10.18502/ijm.v17i3.18827

Aleksanyan Tigran Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Institute of Biology, Yerevan State University, Yerevan, Armenia
Hakobyan Lusine Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Institute of Biology, Yerevan State University, Yerevan, Armenia
Harutyunyan Ruzanna Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Institute of Biology, Yerevan State University, Yerevan, Armenia
Semerjyan Inesa Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Institute of Biology, Yerevan State University, Yerevan, Armenia
Nahapetyan Nelli Department of Obstetrics and Gynecology, Yerevan State Medical University after Mkhitar Heratsi, Yerevan, Armenia
Gharibyan Edita Department of Pregnancy Pathology, Erebuni Medical Center, Yerevan, Armenia
Haertlé Thomas Laboratory of Biopolymères, Interactions, Assemblages (BIA), Institut National de la Recherche Agronomique, Imp. Yvette Cauchois, Nantes, France
Bazukyan Inga Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Institute of Biology, Yerevan State University, Yerevan, Armenia

DOI: https://doi.org/10.18502/ijm.v17i3.18827

Keywords: Antifungal activity; Lacticaseibacillus rhamnosus; Candida albicans; Candidiasis; Bacteriocins

Abstract

Background and Objectives: Probiotics are used for the treatment of yeast infections, they restore the balance in vaginal microbiome, adhere to epithelial cells, compete against pathogenic bacteria, acidify the environment, produce bacteriocins and modulate the immunity. The aim of the study was to investigate the anti-yeast activity (AYA) of the strain Lacticaseiba- cillus rhamnosus R-2002 against different Candida species.

Materials and Methods: From 20 strains of lactic acid bacteria examined, only L. rhamnosus R-2002 strain demonstrated beneficial properties against yeast. The effects of temperature and pH on AYA and its relation to cell wall were revealed by bi-layer agar assay. The connection of AYA to the cell wall was determined with the sonicated cells.

Results: R-2002 inhibited the growth of C. albicans ATCC 10291, C. tropicalis G 31 and C. albicans G4 (both isolated from vaginal samples). R-2002 maintained its AYA between a wide range of pH and its anti-yeast component/s are extracellular. The tested strain demonstrated stability against the high concentrations of progesterone and metronidazole, making it a suit- able candidate for the mitigation of vaginitis.

Conclusion: The present study summarizes all the positive features of the strain R-2002 and its potential as a therapeutic agent in the treatment of candidiasis.