Activity of cefiderocol on extensively drug-resistant Pseudomonas aeruginosa from burn wound infections in Mansoura, Egypt

Rasha  El-Mahdy; Ahmed  Mostafa; Nora El-Tantawy; Raghdaa  Shrief

doi:10.18502/ijm.v17i2.18384

Rasha El-Mahdy Department Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Ahmed Mostafa Department Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Nora El-Tantawy Department Public Health, Faculty of Applied Medical Sciences, Al-Baha University, Al-Baha, Saudi Arabia
Raghdaa Shrief Department Medical Microbiology and Immunology, Faculty of Medicine, Damietta University, Damietta, Egypt

DOI: https://doi.org/10.18502/ijm.v17i2.18384

Keywords: Pseudomonas aeruginosa; Extended detection and response; NDM; Carbapenemase; Burn

Abstract

Background and Objectives: Increased Pseudomonas aeruginosa antibiotic resistance limits treatment options and is as- sociated with a higher level of mortality and mordacity. The purpose of this research was to identify class 1 and 2 integrons, carbapenemase, SHV, and TEM genes in extensively drug-resistant (XDR) P. aeruginosa isolated from infected burns and evaluate their in vitro cefiderocol activity.

Results: From the 110 P. aeruginosa, 54 isolates (49%) were XDR. TEM gene was detected in 35 isolates. Among XDR iso- lates, carbapenemase genes were detected in 31.5%, with NDM being predominant Thirty XDR isolates had class1 integrons. All isolates were sensitive to cefiderocol and its MIC /MIC was 0.5/1.5mg/L (range 0.064-1.5mg/L).

Materials and Methods: By using the disc diffusion method, the antimicrobial susceptibility of 110 P. aeruginosa isolates collected from infected burns were evaluated. XDR P. aeruginosa were screened phenotypically for carbapenemase and extended spectrum β-lactamases (ESBLs) production. Both MIC Test Strip and disc diffusion were employed to test the cefiderocol susceptibility. PCR was used to assess carbapenemase, SHV and TEM genes and integrons class 1 and 2.

Conclusion: Nearly half the P. aeruginosa isolates from burn infections were extensively drug-resistant. Cefiderocol's in vitro activity demonstrated that it is a promising therapy alternative for treating extensively drug-resistant P. aeruginosa in burn patients.