Frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairies and raw milks collected in Yazd province, Iran

Raziyeh  Barzegar-Bafrouei; Bahador  Hajimohammadi; Hengameh  Zandi; Gilda  Eslami; Hossein  Fallahzadeh

doi:10.18502/ijm.v16i6.17248

Raziyeh Barzegar-Bafrouei Department of Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Bahador Hajimohammadi Department of Nutrition, Faculty of Health, Golestan University of Medical Sciences, Gorgan, Iran
Hengameh Zandi Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Gilda Eslami Department of Medical Parasitology and Mycology, Isfahan Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
Hossein Fallahzadeh Departments of Biostatistics and Epidemiology, Research Center for Healthcare Data Modeling, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

DOI: https://doi.org/10.18502/ijm.v16i6.17248

Keywords: Dairy products; invA; Cow’s milk; Salmonella; Antimicrobial resistance

Abstract

Background and Objectives: Salmonellosis is among the most common food-born infections, caused by Salmonella spp. bacteria. Present study has investigated the frequency and antibiotic resistance pattern of Salmonella spp. isolated from tra- ditional dairy products and raw milk supplied in Yazd, Iran.

Materials and Methods: In a cross-sectional study, 350 samples of raw milk and traditional dairy products were randomly collected from July to September 2018. Following culturing the samples, isolates went through biochemical tests for pheno- typic identification. Results were confirmed through PCR technique by targeting invA gene. Antimicrobial susceptibility test was conducted by means of disk diffusion method.

Results: The rate of contamination with Salmonella bacteria was 6.57% in all samples. The PCR assay of all isolates showed that 23 isolates (100%) carried the invA gene. No significant association between the frequency of salmonella spp. and types of dairy and their origin was reported (P>0.05). The highest antibiotic resistance rate among the isolates belonged to tetracy- cline (34.8%) and the highest sensitivity was seen to imipenem, cefepime, and cefotaxime (each 91.3%).

Conclusion: According to our results there has been a rise in multiple drug resistance and contamination rate in traditional dairy products in Yazd province.