Effect of collagen hydrogel containing Lavandula officinalis essential oil nanoemulsion in wound healing of infectious burn

Hossein Teymouri; Mojgan Mohammadimehr; Mohammad Ahanjan; Somayeh Sheidaei; Majid Saeedi; Amir Mellati

doi:10.18502/ijm.v16i3.15795

Hossein Teymouri Infectious Diseases Research Center, Aja University of Medical Sciences, Tehran, Iran
Mojgan Mohammadimehr Infectious Diseases Research Center, Aja University of Medical Sciences, Tehran, Iran
Mohammad Ahanjan Department of Medical Bacteriology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Somayeh Sheidaei Department of Pathology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Majid Saeedi Department of Pharmaceutics, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Amir Mellati Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran

DOI: https://doi.org/10.18502/ijm.v16i3.15795

Keywords: Lavandula; Wound healing; Collagen; Hydrogels; Burns

Abstract

Background and Objectives: The main cause of mortality in burn patients is infection from burns. Drug-resistant bacteria are the main causes of wound infection, so alternative antibiotic therapies hold significant importance. The objective of this study was to examine the impact of a collagen hydrogel that contains a nanoemulsion of Lavandula essential oil on the heal- ing process of infected burn wounds.

Materials and Methods: In this experimental study, 20 rats were randomly divided after applying burns with a 10 mm diameter hot plate and infecting the wounds with multidrug-resistant Pseudomonas aeruginosa into four groups, including a positive control, a negative control, the first experiment (collagen hydrogel), and the second experiment (collagen hydrogel containing Lavandula essential oil nanoemulsion). On the 4th, 11th, and 18th days, tissue samples were taken for pathology studies. The important parameters in burn wound healing with hematoxylin and eosin and Masson's trichrome staining meth- ods were investigated and scored according to Abramov’s method.

Results: Based on the pathology findings, experimental groups 1 and 2 compared to the negative and positive control groups were effective in rat infection wound healing. The hydrogel scaffold in the experimental groups increased fibroblasts and an- giogenesis compared to the control groups. Epithelization was noticed only in the hydrogel group containing nanoemulsion.

Conclusion: The study findings suggest that the use of collagen hydrogel with Lavandula essential oil nanoemulsion has potential as a wound dressing. This is because it has the potential to effectively promote healing and act as an antibacterial agent to prevent infections.