Bacillus megaterium RTS1 enhances resistance of Lycopersicon esculentum to salinity stress through the improvement of antioxidant defenses

Shiva Yavarian; Parvaneh Jafari; Neda Akbari

doi:10.18502/ijm.v15i5.13874

Shiva Yavarian Department of Microbiology, Faculty of Sciences, Arak Branch, Islamic Azad University, Arak, Iran
Parvaneh Jafari Department of Microbiology, Faculty of Sciences, Arak Branch, Islamic Azad University, Arak, Iran
Neda Akbari Department of Microbiology, Faculty of Sciences, Arak Branch, Islamic Azad University, Arak, Iran

DOI: https://doi.org/10.18502/ijm.v15i5.13874

Keywords: Plant growth-promoting bacteria (PGPBs); Bacillus megaterium; Lycopersicon esculentum; Salinity stress

Abstract

Background and Objectives: Plant growth-promoting bacteria (PGPB) may reduce the negative effects of salinity stress. The aim of this study was to optimize Bacillus megaterium RTS1 and characterize the effect of the PGPB on the physiolog- ical characteristics of tomato (Lycopersicon esculentum).

Materials and Methods: The Central composite design (CCD) of response surface methodology (RSM) was used to opti- mize Bacillus megaterium RTS1 to produce maximum cell biomass and spores. Then the effect of the PGPB on the physi- ological characteristics of tomato (Lycopersicon esculentum), including membrane stability, leaf relative water content per- centage, anthocyanin and carotenoids content, chlorophyll photosynthetic parameters, sugar and starch level, superoxide anion and antioxidant activity under salt stress conditions. The NFB medium was inoculated with 5% bacterial culture and the fermentation was carried out in a 10-lit fermenter.

Results: After optimization, the amount of cell biomass by the model was 9.45 log10 CFUs/mL, which showed a 1.2-fold increase compared to the non-optimized medium. Usage of bacteria under the optimal conditions of the culture medium may increase the stability of the membrane and improve the relative water content. Bacteria were able to prevent the excessive increase of anthocyanins. Oxidative stress led to an increase in the content of chlorophyll a, while causing the degradation of chlorophyll b. Bacterial inoculation led to an increase in the level of sugar and starch compared to the control. PGPB showed an increasing effect on the amount of superoxide anion production and caused a significant increase in the antioxidant activity under salinity stress conditions.

Conclusion: The PGPB can be a promising way to boost physiological characteristics of tomato plant under salinity stress. Also, sporulation capacity of Bacillus megaterium with high bacterial cell density in fermenter produce a sustainable product for tomato plants.