Glucomannan enhanced the macrophage activity in exposure to methicillin-resistant Staphylococcus aureus (MRSA): in-vitro study

Melika Khanzadeh Tehrani; Mohammad Hossein Yazdi; Mohammad Reza Pourmand

doi:10.18502/ijm.v15i4.13510

Melika Khanzadeh Tehrani Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Hossein Yazdi Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Reza Pourmand Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/ijm.v15i4.13510

Keywords: Glucomannan; Macrophage; Methicillin-resistant Staphylococcus aureus; Inducible nitric oxide synthase; Tumor necrosis factor-alph

Abstract

Background and Objectives: The increasing number of methicillin-resistant Staphylococcus aureus persuade the need for preventive measures. Glucomannan is a polysaccharide choice for developing immunological strategies. This study aimed to investigate changes in gene expression and phagocytic activity of macrophage cells in the presence of glucomannan.

Materials and Methods: The effect of different concentrations of glucomannan (25, 50, and 100 µg/mL) on the phagocytic activity of macrophage cells was measured using the colony count method. The expression of Tumor Necrosis Factor-alpha (TNF-α) and Inducible Nitric Oxide Synthase (iNOS) genes was evaluated by Real-Time PCR.

Results: The concentrations of glucomannan significantly reduced the bacterial Colony-Forming Unit (CFU) and increased the phagocytic activity of macrophage cells. The maximum effect of glucomannan on iNOS and TNF-Α genes expression was 100 µg/mL.

Conclusion: Glucomannan should be considered an adjuvant that stimulates the immune system. It may increase the expres- sion of TNF-α and iNOS genes and the phagocytic activity of macrophage cells against methicillin-resistant Staphylococcus aureus.