Prevalence of Gardnerella vaginalis infection and antibiotic resistance pattern of isolates of gynecology clinic patients at Shahriar Noor Hospital from January to June 2020 by PCR and culture methods

Saghi Rashidifar; Naser Harzandi; Sahar Honarmand Jahromi; Mohammad Javad Gharavi

doi:10.18502/ijm.v15i4.13505

Saghi Rashidifar Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
Naser Harzandi Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
Sahar Honarmand Jahromi Department of Microbiology, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
Mohammad Javad Gharavi Department of Parasitology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/ijm.v15i4.13505

Keywords: Antibiotic resistance; Bacterial vaginosis; Gardnerella vaginalis; Prevalence; Polymerase chain reaction

Abstract

Background and Objectives: Gardnerella vaginalis is one of the most important causes of prevalent genital infections that pose serious risks. This study aimed to determine the prevalence of Gardnerella vaginalis and antibiotic resistance pattern of isolates of patients referred to the gynecology clinic of Shahriar Noor Hospital by PCR and culture methods.

Materials and Methods: The study was conducted on 500 patients who had suffered from a vaginal infection. The demo- graphic data of patients were studied. For diagnosis of Gardnerella vaginalis isolates, cultivation in anaerobic conditions, biochemical tests, PCR and Gardnerella vaginalis antibiotic susceptibility test to metronidazole and clindamycin were per- formed. Data analysis was performed utilizing SPSS statistical software version 19 and the Chi-square test.

Results: Among the 500 patients, 173 were diagnosed with Gardnerella vaginitis. There was a significant relationship be- tween age group, level of education, and contraceptive method with Gardnerella vaginosis incidence. Performing antibiotic susceptibility tests showed that the resistance of Gardnerella vaginalis isolated strains to metronidazole and clindamycin was 86.12% and 17.34%, respectively.

Conclusion: The high prevalence of Gardnerella vaginalis infections confirms the critical role of the bacterium in the oc- currence of bacterial vaginosis. Therefore, it is necessary to check the prevalence of bacterial infections to recommend the correct medical treatment in different societies.