Molecular characterization of typing and subtyping of Staphylococcal cassette chromosome SCCmec types I to V in methicillin-resistant Staphylococcus aureus from clinical isolates from COVID-19 patients

Vivekanand Jadhav; Meenakshi Bhakare; Arundhuti Paul; Sumedh Deshpande; Madhusmita Mishra; Anjali  Apte-Deshpande; Neetu Gupta; Savita V Jadhav

doi:10.18502/ijm.v15i4.13502

Vivekanand Jadhav Department of Microbiology, LNCT Medical College and Sewakunj Hospital, Madhya Pradesh, India
Meenakshi Bhakare Department of Respiratory Medicine, Symbiosis Medical College for Women (SMCW) and Symbiosis University Hospital and Research Centre (SUHRC), Maharashtra, India
Arundhuti Paul Department of Microbiology, Govind Ballabh Pant Institute of Postgraduate Medical Education and Research, New Delhi, India
Sumedh Deshpande Department of Biotechnology, Central Dogma Pvt. Ltd, Maharashtra, India
Madhusmita Mishra Department of Biotechnology, Central Dogma Pvt. Ltd, Maharashtra, India
Anjali Apte-Deshpande Department of Biotechnology, Central Dogma Pvt. Ltd, Maharashtra, India
Neetu Gupta Department of Microbiology, Symbiosis Medical College for Women (SMCW) and Symbiosis University Hospital and Research Centre (SUHRC), Maharashtra, India
Savita V Jadhav Department of Microbiology, LNCT Medical College and Sewakunj Hospital, Madhya Pradesh, India

DOI: https://doi.org/10.18502/ijm.v15i4.13502

Keywords: Methicillin-resistant Staphylococcus aureus; SSCmec types and subtypes I; II; III; Iva; V; Staphylococcal cas- sette chromosome mec (SCCmec)

Abstract

Background and Objectives: Methicillin resistance is acquired by the bacterium due to mecA gene which codes for pen- icillin-binding protein (PBP2a) having low affinity for β-lactam antibiotics. mecA gene is located on a mobile genetic el- ement called staphylococcal cassette chromosome mec (SCCmec). SCCmec genomic island comprises two site-specific recombinase genes namely ccrA and ccrB [cassette chromosome recombinase] accountable for mobility. Currently, SCCmec elements are classified into types I, II, III, IV and V based on the nature of the mec and ccr gene complexes and are further classified into subtypes according to variances in their J region DNA. SSCmec type IV has been found in community-ac- quired isolates with various genetic backgrounds. The present study was undertaken to categorize the types of SCCmec types and subtypes I, II, III, IVa, b, c, d, and V and PVL genes among clinical MRSA isolates from COVID-19 confirmed cases.

Materials and Methods: Based on the Microbiological and Molecular (mecA gene PCR amplification) confirmation of MRSA isolated from 500 MRSA SCCmec clinical samples, 144 cultures were selected for multiplex analysis. The multiplex PCR method developed by Zhang et al. was adapted with some experimental alterations to determine the specific type of these isolates.

Results: Of the total 500 MRSA, 144 MRSA (60 were CA-MRSA and 84 were HA-MRSA) were selected for characteriza- tion of novel multiplex PCR assay for SSCmec Types I to V in MRSA. Molecular characterization of multiplex PCR analysis revealed results compare to the phenotypic results. Of the 60 CA-MRSA; in 56 MRSA strains type IVa was found and signifi- cantly defined as CA-MRSA while 4 strains showed mixed gens subtypes. Type II, III, IA, and V were present in overall 84 HA-MRSA. Molecular subtyping was significantly correlated to define molecularly as CA-MRSA and HA-MRSA however 15 (10%) strains showed mixed genes which indicates the alarming finding of changing epidemiology of CA-MRSA and HA-MRSA as well.

Conclusion: We have all witnessed of COVID-19 pandemic, and its mortality was mostly associated with co-morbid con- ditions and secondary infections of MDR pathogens. Rapid detections of causative agents of these superbugs with their changing epidemiology by investing in typing and subtyping clones are obligatory. We have described an assay designed for targeting SSCmec types and subtypes I, II, III, IVa,V according to the current updated SCCmec typing system. Changing patterns of molecular epidemiology has been observed by this newly described assay.