In vitro antifungal potency of the moronecidin-like peptide against Candida albicans, Candida glabrata, and Candida tropicalis

Nasrin Amirrajab; Seyed Amin  Ayatollahi Mousavi; Behrooz Taheri; Samira Salari; Noor Ali Ahmadi Sarsahra

doi:10.18502/ijm.v15i3.12907

Nasrin Amirrajab Department of Laboratory Sciences, School of Allied Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Seyed Amin Ayatollahi Mousavi Department of Parasitology and Mycology, School of Medicine, Medical Mycology Research Center, Kerman University of Medical Sciences, Kerman, Iran
Behrooz Taheri Department of Laboratory Sciences, School of Allied Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Samira Salari Department of Parasitology and Mycology, School of Medicine, Medical Mycology Research Center, Kerman University of Medical Sciences, Kerman, Iran
Noor Ali Ahmadi Sarsahra Department of Parasitology and Mycology, School of Medicine, Medical Mycology Research Center, Kerman University of Medical Sciences, Kerman, Iran

DOI: https://doi.org/10.18502/ijm.v15i3.12907

Keywords: In vitro; Fungi; Antimicrobial peptides; Cytotoxicity

Abstract

Background and Objectives: The aim of this study was to investigate the in vitro antifungal potency of the moronecidin-like peptide against Candida albicans, Candida glabrata, and Candida tropicalis.

Materials and Methods: To evaluate the antifungal effect of moronecidin-like peptide, the protocol presented in CLSI M27-A3 and CLSI M27-S4 was used and the minimum inhibitory concentration was determined.

Results: The minimum inhibitory effect of moronecidin-like peptide composition was 8 µg/ml for Candida tropicalis and Candida albicans and 32 µg/ml for Candida glabrata. The MIC of nystatin was determined to be 1.25 µg/ml for Candida glabrata and Candida albicans and 0.625 µg/ml for Candida tropicalis strains. The MFC composition of the moroneci- din-like peptide was determined for Candida tropicalis and Candida albicans strains 8 µg/ml and for Candida glabrata strain 64 µg/ml. The results of cytotoxicity and hemolysis of the moronecidin peptide test on macrophage showed that moronecidin peptide has no cytotoxicity and toxicity properties.

Conclusion: According to the results of the present study, the moronecidin-like peptide could be a new strategy in the treat- ment of infections caused by Candida strains. The discovery of the exact mechanism of which requires extensive clinical studies in this field.